Lamin B1: a novel biomarker in adult and pediatric adrenocortical carcinoma

in Endocrine-Related Cancer
Authors:
Yihao Chen Department of Andrology, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, China
Department of Urology, Huizhou Municipal Central Hospital, Huizhou, Guangdong, China

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Jiahong Chen Department of Urology, Huizhou Municipal Central Hospital, Huizhou, Guangdong, China

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Yongcheng Shi Department of Urology, Huizhou Municipal Central Hospital, Huizhou, Guangdong, China

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Xiaohui Ling Reproductive Medicine Center, Huizhou Municipal Central Hospital, Huizhou, Guangdong, China

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Shumin Fang Science Research Center, Huizhou Municipal Central Hospital, Huizhou, Guangdong, China

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Chuanfan Zhong Department of Urology, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, China

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Fengping Liu State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Taipa, Macau, China

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Weide Zhong State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Taipa, Macau, China
Department of Urology, Guangdong Key Laboratory of Clinical Molecular Medicine and Diagnostics, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, China
Department of Urology, Minimally Invasive Surgery Center, The First Affiliated Hospital of Guangzhou Medical University, Guangdong Key Laboratory of Urology, Guangzhou Institute of Urology, Guangzhou, Guangdong

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Xuecheng Bi Department of Urology, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, China

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Zhong Dong Department of Urology, Huizhou Municipal Central Hospital, Huizhou, Guangdong, China

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Jianming Lu Department of Andrology, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, China
Department of Urology, Guangdong Key Laboratory of Clinical Molecular Medicine and Diagnostics, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, China

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https://orcid.org/0000-0002-3794-641X

Correspondence should be addressed to Z Dong or J Lu: hzdongzhong@126.com or louiscfc8@gmail.com

*(Y Chen, J Chen and Y Shi contributed equally to this work)

DOI:
https://doi.org/10.1530/ERC-23-0178
Volume/Issue:
Volume 31: Issue 3
Article Type:
Research Article
Article ID:
e230178
Online Publication Date:
08 Feb 2024
Copyright:
© the author(s) 2024
Restricted access
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Adrenocortical carcinoma (ACC) is a malignancy with a poor prognosis and high mortality rate. A high tumor mutational burden (TMB) has been found to be associated with poor prognosis in ACC. Thus, exploring ACC biomarkers based on TMB holds significant importance for patient risk stratification. In our research, we utilized weighted gene coexpression network analysis and an assay for transposase-accessible chromatin with high-throughput sequencing to identify genes associated with TMB. Through the comprehensive analysis of various public datasets, Lamin B1 (LMNB1) was identified as a biomarker associated with a high TMB and low chromatin accessibility. Immunohistochemical staining demonstrated high expression of LMNB1 in ACC compared to noncancerous tissues. Functional enrichment analyses revealed that the function of LMNB1 is associated with cell proliferation and division. Furthermore, cell assays suggested that LMNB1 promotes tumor proliferation and invasion. In addition, mutation analysis suggested that the high expression of LMNB1 is associated with TP53 mutations. Additionally, LMNB1 was highly expressed in the vast majority of solid tumors across cancers. In our immune analysis, we discovered that the high expression of LMNB1 might suppress the infiltration of CD8+ T cells in the ACC microenvironment. In summary, LMNB1 is a predictive factor for the poor prognosis of adult and pediatric ACC. Its high expression in ACC is positively associated with high TMB and lower chromatin accessibility, and it promotes ACC cell proliferation and invasion. Therefore, LMNB1 holds promise as a novel biomarker and potential therapeutic target for ACC.

Supplementary Materials

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Print ISSN:
1351-0088
Online ISSN:
1479-6821
Copyright:
© the author(s) 2024
Article ID:
e230178
Received Date:
13 Jun 2023
Accepted Date:
15 Jan 2024
Print Publication Date:
01 Mar 2024
Online Publication Date:
08 Feb 2024
Keywords:
adrenocortical carcinoma; ATAC-seq; TMB; LMNB1; prognosis

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