Search Results

You are looking at 121 - 130 of 431 items for :

  • Refine by access: All content x
Clear All
M Muratori
Search for other papers by M Muratori in
Google Scholar
PubMed
Close
,
I Nicoletti
Search for other papers by I Nicoletti in
Google Scholar
PubMed
Close
,
G B Vannelli
Search for other papers by G B Vannelli in
Google Scholar
PubMed
Close
,
M Luconi
Search for other papers by M Luconi in
Google Scholar
PubMed
Close
,
E Macorsini
Search for other papers by E Macorsini in
Google Scholar
PubMed
Close
,
M Serio
Search for other papers by M Serio in
Google Scholar
PubMed
Close
,
G Forti
Search for other papers by G Forti in
Google Scholar
PubMed
Close
, and
M Maggi
Search for other papers by M Maggi in
Google Scholar
PubMed
Close

Abstract

We report evidence indicating that the isoflavone genistein induces a dose-dependent antiproliferative effect in the human uterine adenocarcinoma cell lines HEC-1A, HEC-1B, AN3 CA and RL95-2. Cell growth inhibition resulted from a partial G2/M block and from the appearance of a hypodiploid DNA peak (reduction in nuclear DNA content), suggestive of apoptosis. In HEC-1A cells, we found that both cell cycle impairment and the appearance of a hypodiploid DNA peak were time-dependent, triggered by similar concentrations of the isoflavone and not affected by the presence of serum in the culture medium. However, while the genistein-induced cell cycle'arrest was fully reversible, the appearance of the hypodiploid DNA peak was not. To verify whether the appearance of a hypodiploid DNA peak corresponded to apoptosis, we used in situ end labelling (ISEL) and transmission electron microscopy (TEM) in HEC-1A cells. We found that a 48-h treatment with genistein induced ISEL positivity only in a minority of cells, while at 72 h the majority of cells were labelled. At this time TEM showed the typical ultrastructural features of apoptosis, including apoptotic bodies.

Because genistein inhibited tyrosine kinase (TK) and topoisomerase (Topo) II activity in HEC-1A cells and its effects were mimicked by structurally unrelated TK and Topo II inhibitors, we speculate that interactions with TK and Topo II are relevant for the antiproliferative effect of genistein. Conversely, the antiproliferative effect of genistein seems to be independent of its oestrogenic activity. Our data indicate that genistein inhibits the growth of uterine adenocarcinoma cell lines by inducing cell cycle arrest and apoptosis.

Endocrine-Related Cancer (1997) 4 203-218

Restricted access
Riccardo Ponzone Gynaecological Oncology, Institute for Cancer Research and Treatment (IRCC) and ASO Ordine Mauriziano, University of Turin, Turin 10060, Italy

Search for other papers by Riccardo Ponzone in
Google Scholar
PubMed
Close
,
Paola Mininanni Gynaecological Oncology, Institute for Cancer Research and Treatment (IRCC) and ASO Ordine Mauriziano, University of Turin, Turin 10060, Italy

Search for other papers by Paola Mininanni in
Google Scholar
PubMed
Close
,
Elisa Cassina Gynaecological Oncology, Institute for Cancer Research and Treatment (IRCC) and ASO Ordine Mauriziano, University of Turin, Turin 10060, Italy

Search for other papers by Elisa Cassina in
Google Scholar
PubMed
Close
,
Francesca Pastorino Gynaecological Oncology, Institute for Cancer Research and Treatment (IRCC) and ASO Ordine Mauriziano, University of Turin, Turin 10060, Italy

Search for other papers by Francesca Pastorino in
Google Scholar
PubMed
Close
, and
Piero Sismondi Gynaecological Oncology, Institute for Cancer Research and Treatment (IRCC) and ASO Ordine Mauriziano, University of Turin, Turin 10060, Italy

Search for other papers by Piero Sismondi in
Google Scholar
PubMed
Close

) was beyond the understanding and expectations of their proponents and opened the avenue of oestrogen deprivation as a means to block tumour growth. The selective efficacy of ovariectomy and adrenalectomy for respectively pre- and postmenopausal

Free access
Douglas A Gibson MRC Centre for Reproductive Health The University of Edinburgh, The Queen's Medical Research Institute, 47 Little France Crescent, Edinburgh EH16 4TJ, UK

Search for other papers by Douglas A Gibson in
Google Scholar
PubMed
Close
,
Ioannis Simitsidellis MRC Centre for Reproductive Health The University of Edinburgh, The Queen's Medical Research Institute, 47 Little France Crescent, Edinburgh EH16 4TJ, UK

Search for other papers by Ioannis Simitsidellis in
Google Scholar
PubMed
Close
,
Frances Collins MRC Centre for Reproductive Health The University of Edinburgh, The Queen's Medical Research Institute, 47 Little France Crescent, Edinburgh EH16 4TJ, UK

Search for other papers by Frances Collins in
Google Scholar
PubMed
Close
, and
Philippa T K Saunders MRC Centre for Reproductive Health The University of Edinburgh, The Queen's Medical Research Institute, 47 Little France Crescent, Edinburgh EH16 4TJ, UK

Search for other papers by Philippa T K Saunders in
Google Scholar
PubMed
Close

divided into two subtypes: oestrogen-dependent type I and the less common, but clinically more aggressive, oestrogen-independent type II ( Emons et al . 2000 ). Approximately 95% of endometrial tumours are adenocarcinomas arising due to malignant

Free access
R I Nicholson
Search for other papers by R I Nicholson in
Google Scholar
PubMed
Close
,
J M W Gee
Search for other papers by J M W Gee in
Google Scholar
PubMed
Close
,
A Harris
Search for other papers by A Harris in
Google Scholar
PubMed
Close
, and
E Anderson
Search for other papers by E Anderson in
Google Scholar
PubMed
Close

influence how we proceed in the future. Session 1 focused around oestrogen receptor-α (ERα) as an old target that has recently spawned the exciting third generation of aromatase inhibitors and where new knowledge of the molecular actions of the ER is

Free access
Andrea Nicolini Department of Oncology, Transplantations and New Technologies in Medicine, University of Pisa, Pisa, Italy

Search for other papers by Andrea Nicolini in
Google Scholar
PubMed
Close
,
Paola Ferrari Department of Oncology, Transplantations and New Technologies in Medicine, University of Pisa, Pisa, Italy

Search for other papers by Paola Ferrari in
Google Scholar
PubMed
Close
,
Giuseppe Rossi Unit of Epidemiology and Biostatistics, Institute of Clinical Physiology, National Council of Research, Pisa, Italy

Search for other papers by Giuseppe Rossi in
Google Scholar
PubMed
Close
, and
Angelo Carpi Department of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy

Search for other papers by Angelo Carpi in
Google Scholar
PubMed
Close

apoptosis have been identified ( Dou et al. 2014 ). Many reports have suggested a relevant role of IFNγ/STAT1/IRF-1 axis in the endocrine resistance of oestrogen receptor (ER)-positive breast cancer cells ( Clarke et al. 2009 , Ning et al. 2010

Free access
H E Jones
Search for other papers by H E Jones in
Google Scholar
PubMed
Close
,
J M W Gee
Search for other papers by J M W Gee in
Google Scholar
PubMed
Close
,
K M Taylor
Search for other papers by K M Taylor in
Google Scholar
PubMed
Close
,
D Barrow
Search for other papers by D Barrow in
Google Scholar
PubMed
Close
,
H D Williams
Search for other papers by H D Williams in
Google Scholar
PubMed
Close
,
M Rubini
Search for other papers by M Rubini in
Google Scholar
PubMed
Close
, and
R I Nicholson
Search for other papers by R I Nicholson in
Google Scholar
PubMed
Close

first and extremely successful targeted therapies in oncology could considered to be the development of anti-hormone therapies for breast and prostate cancer which blocked the action of the oestrogen receptor (ER) and androgen receptor respectively, with

Free access
Keely M McNamara
Search for other papers by Keely M McNamara in
Google Scholar
PubMed
Close
,
Nicole L Moore Department of Pathology Tohoku University School of Medicine, Miyagi, Sendai, Japan

Dame Roma Mitchell Cancer Research Laboratories Discipline of Medicine, The University of Adelaide and Hanson Institute, DX 650801, Adelaide, South Australia 5005, Australia

Search for other papers by Nicole L Moore in
Google Scholar
PubMed
Close
,
Theresa E Hickey Department of Pathology Tohoku University School of Medicine, Miyagi, Sendai, Japan

Dame Roma Mitchell Cancer Research Laboratories Discipline of Medicine, The University of Adelaide and Hanson Institute, DX 650801, Adelaide, South Australia 5005, Australia

Search for other papers by Theresa E Hickey in
Google Scholar
PubMed
Close
,
Hironobu Sasano
Search for other papers by Hironobu Sasano in
Google Scholar
PubMed
Close
, and
Wayne D Tilley Department of Pathology Tohoku University School of Medicine, Miyagi, Sendai, Japan

Dame Roma Mitchell Cancer Research Laboratories Discipline of Medicine, The University of Adelaide and Hanson Institute, DX 650801, Adelaide, South Australia 5005, Australia

Search for other papers by Wayne D Tilley in
Google Scholar
PubMed
Close

masculinising side effects in some women and the concurrent development of targeted anti-oestrogenic therapies. With recent insights into the molecular heterogeneity of breast cancers, intracrine steroid metabolism and mechanisms of anti-oestrogen therapy

Free access
Caroline Wilson Academic Unit of Clinical Oncology, Weston Park Hospital, University of Sheffield, Sheffield, UK

Search for other papers by Caroline Wilson in
Google Scholar
PubMed
Close
,
Hannah Brown Department of Oncology and Metabolism, University of Sheffield, Sheffield, UK

Search for other papers by Hannah Brown in
Google Scholar
PubMed
Close
, and
Ingunn Holen Department of Oncology and Metabolism, University of Sheffield, Sheffield, UK

Search for other papers by Ingunn Holen in
Google Scholar
PubMed
Close

the cancer cells from an epithelial to a more invasive mesenchymal phenotype, which may promote the spread of these cells to the bone ( El-Haibi et al . 2012 ). In a cohort of patients with oestrogen receptor negative (ER−) breast cancers, primary

Free access
Joanna M Day Department of Endocrinology and Metabolic Medicine and Sterix Ltd, Imperial College London, St Mary's Hospital, 2nd Floor, Mint Wing, Winsland Street, London W2 1NY, UK

Search for other papers by Joanna M Day in
Google Scholar
PubMed
Close
,
Helena J Tutill Department of Endocrinology and Metabolic Medicine and Sterix Ltd, Imperial College London, St Mary's Hospital, 2nd Floor, Mint Wing, Winsland Street, London W2 1NY, UK

Search for other papers by Helena J Tutill in
Google Scholar
PubMed
Close
,
Atul Purohit Department of Endocrinology and Metabolic Medicine and Sterix Ltd, Imperial College London, St Mary's Hospital, 2nd Floor, Mint Wing, Winsland Street, London W2 1NY, UK

Search for other papers by Atul Purohit in
Google Scholar
PubMed
Close
, and
Michael J Reed Department of Endocrinology and Metabolic Medicine and Sterix Ltd, Imperial College London, St Mary's Hospital, 2nd Floor, Mint Wing, Winsland Street, London W2 1NY, UK

Search for other papers by Michael J Reed in
Google Scholar
PubMed
Close

Introduction Steroid-dependent diseases Breast cancer, a major cause of death in both European and American women, occurs most frequently in post-menopausal women. After menopause, a low level of oestrogen is produced, mainly from the local

Free access
V Craig Jordan Departments of Breast Medical Oncology and Molecular and Cellular Oncology, MD Anderson Cancer Center, Houston, Texas 77030, USA

Search for other papers by V Craig Jordan in
Google Scholar
PubMed
Close

extraordinary extent of tumour regression in perhaps 1% of postmenopausal cases (with oestrogen) has always be regarded as of major theoretical importance and it is a matter for some disappointment that so much of the underlying mechanism continues to elude us

Free access