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M Principe Cancer Research Centre of Lyon (CRCL), INSERM U1052, CNRS UMR5286, Claude Bernard University, Lyon, France

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M Chanal Cancer Research Centre of Lyon (CRCL), INSERM U1052, CNRS UMR5286, Claude Bernard University, Lyon, France

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V Karam Cancer Research Centre of Lyon (CRCL), INSERM U1052, CNRS UMR5286, Claude Bernard University, Lyon, France

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A Wierinckx Cancer Research Centre of Lyon (CRCL), INSERM U1052, CNRS UMR5286, Claude Bernard University, Lyon, France
ProfilXpert, Lyon, France

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I Mikaélian Cancer Research Centre of Lyon (CRCL), INSERM U1052, CNRS UMR5286, Claude Bernard University, Lyon, France

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R Gadet Cancer Research Centre of Lyon (CRCL), INSERM U1052, CNRS UMR5286, Claude Bernard University, Lyon, France

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C Auger Cancer Research Centre of Lyon (CRCL), INSERM U1052, CNRS UMR5286, Claude Bernard University, Lyon, France

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V Raverot Laboratoire d’Hormonologie, Centre de Biologie et de Pathologie Est, Hospices Civils de Lyon, Lyon, France

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E Jouanneau Service de Neurochirurgie, Groupement Hospitalier Est, Hospices Civils de Lyon, Bron, France
Faculté de Médecine Lyon Est, Université Lyon 1, Lyon, France

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A Vasiljevic Faculté de Médecine Lyon Est, Université Lyon 1, Lyon, France
Department of Pathology, Groupement Hospitalier EST, Hospices Civils de Lyon, University of Lyon, Lyon, France

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A Hennino Cancer Research Centre of Lyon (CRCL), INSERM U1052, CNRS UMR5286, Claude Bernard University, Lyon, France

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G Raverot Cancer Research Centre of Lyon (CRCL), INSERM U1052, CNRS UMR5286, Claude Bernard University, Lyon, France
Department of Pathology, Groupement Hospitalier EST, Hospices Civils de Lyon, University of Lyon, Lyon, France
Department of Endocrinology, Reference Center for Rare Pituitary Disease (HYPO), Groupement Hospitalier EST, Hospices Civils de Lyon, University of Lyon, Lyon, France

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P Bertolino Cancer Research Centre of Lyon (CRCL), INSERM U1052, CNRS UMR5286, Claude Bernard University, Lyon, France

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-Cruz Biotechnology), P-Smad2 (Rabbit, Chemicon International, USA), cleaved caspase3 (Rabbit, Cell Signaling Technology). Phase contrast and fluorescence images were acquired on an Eclipse-NiE NIKON microscope and analysed using NIS-Elements Software. ALK7 expression

Article Type:
Research Article
DOI:
https://doi.org/10.1530/ERC-18-0082
Volume/Issue:
Volume 25: Issue 9
Free access
Paola De Marco
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Enrica Romeo
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Adele Vivacqua
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Roberta Malaguarnera Department of Pharmacy Health and Nutritional Sciences, University of Calabria, 87036 Rende (CS), Italy

Regional Hospital Cosenza, Italy

Endocrinology Department of Health, University Magna Graecia of Catanzaro, Catanzaro, Italy

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Sergio Abonante Department of Pharmacy Health and Nutritional Sciences, University of Calabria, 87036 Rende (CS), Italy

Regional Hospital Cosenza, Italy

Endocrinology Department of Health, University Magna Graecia of Catanzaro, Catanzaro, Italy

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Francesco Romeo Department of Pharmacy Health and Nutritional Sciences, University of Calabria, 87036 Rende (CS), Italy

Regional Hospital Cosenza, Italy

Endocrinology Department of Health, University Magna Graecia of Catanzaro, Catanzaro, Italy

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Vincenzo Pezzi
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Antonino Belfiore Department of Pharmacy Health and Nutritional Sciences, University of Calabria, 87036 Rende (CS), Italy

Regional Hospital Cosenza, Italy

Endocrinology Department of Health, University Magna Graecia of Catanzaro, Catanzaro, Italy

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Marcello Maggiolini
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6000 Advanced Fluorescence Imaging System supported by the quantification and image processing software Leica Application Suite Advanced Fluorescence (Leica Microsystems CMS, GbH Mannheim, Germany) were used for evaluation of experiments. Migration

Article Type:
Research Article
DOI:
https://doi.org/10.1530/ERC-14-0245
Volume/Issue:
Volume 21: Issue 5
Free access
Juan Pablo Petiti Instituto de Investigaciones en Ciencias de la Salud (INICSA), Centro de Microscopía Electrónica-Facultad de Ciencias Médicas, CONICET, Universidad Nacional de Córdoba, Córdoba, Argentina

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Liliana del Valle Sosa Instituto de Investigaciones en Ciencias de la Salud (INICSA), Centro de Microscopía Electrónica-Facultad de Ciencias Médicas, CONICET, Universidad Nacional de Córdoba, Córdoba, Argentina

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Florencia Picech Instituto de Investigaciones en Ciencias de la Salud (INICSA), Centro de Microscopía Electrónica-Facultad de Ciencias Médicas, CONICET, Universidad Nacional de Córdoba, Córdoba, Argentina

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Gabriela Deisi Moyano Crespo Instituto de Investigaciones en Ciencias de la Salud (INICSA), Centro de Microscopía Electrónica-Facultad de Ciencias Médicas, CONICET, Universidad Nacional de Córdoba, Córdoba, Argentina

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Jean Zander Arevalo Rojas Instituto de Investigaciones en Ciencias de la Salud (INICSA), Centro de Microscopía Electrónica-Facultad de Ciencias Médicas, CONICET, Universidad Nacional de Córdoba, Córdoba, Argentina

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Pablo Anibal Pérez Instituto de Investigaciones en Ciencias de la Salud (INICSA), Centro de Microscopía Electrónica-Facultad de Ciencias Médicas, CONICET, Universidad Nacional de Córdoba, Córdoba, Argentina

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Carolina Beatriz Guido Instituto de Investigaciones en Ciencias de la Salud (INICSA), Centro de Microscopía Electrónica-Facultad de Ciencias Médicas, CONICET, Universidad Nacional de Córdoba, Córdoba, Argentina

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Carolina Leimgruber Instituto de Investigaciones en Ciencias de la Salud (INICSA), Centro de Microscopía Electrónica-Facultad de Ciencias Médicas, CONICET, Universidad Nacional de Córdoba, Córdoba, Argentina

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María Eugenia Sabatino Instituto de Investigaciones en Ciencias de la Salud (INICSA), Centro de Microscopía Electrónica-Facultad de Ciencias Médicas, CONICET, Universidad Nacional de Córdoba, Córdoba, Argentina

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Pedro García Instituto de Radioterapia, Fundación Marie Curie, Córdoba, Argentina

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Verónica Bengio Servicio de Patología, Hospital Córdoba, Córdoba, Argentina

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Francisco Roque Papalini Servicio de Neurocirugía, Hospital Córdoba, Córdoba, Argentina

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Paula Estario Servicio de Endocrinología, Hospital Córdoba, Córdoba, Argentina

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Celina Berhard Servicio de Patología, Clínica Reina Fabiola, Córdoba, Argentina

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Marcos Villarreal Instituto de Investigaciones en Físico-Química de Córdoba (INFIQC), Facultad de Ciencias Químicas, CONICET, Universidad Nacional de Córdoba, Córdoba, Argentina

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Silvina Gutiérrez Instituto de Investigaciones en Ciencias de la Salud (INICSA), Centro de Microscopía Electrónica-Facultad de Ciencias Médicas, CONICET, Universidad Nacional de Córdoba, Córdoba, Argentina

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Ana Lucía De Paul Instituto de Investigaciones en Ciencias de la Salud (INICSA), Centro de Microscopía Electrónica-Facultad de Ciencias Médicas, CONICET, Universidad Nacional de Córdoba, Córdoba, Argentina

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Jorge Humberto Mukdsi Instituto de Investigaciones en Ciencias de la Salud (INICSA), Centro de Microscopía Electrónica-Facultad de Ciencias Médicas, CONICET, Universidad Nacional de Córdoba, Córdoba, Argentina

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Alicia Inés Torres Instituto de Investigaciones en Ciencias de la Salud (INICSA), Centro de Microscopía Electrónica-Facultad de Ciencias Médicas, CONICET, Universidad Nacional de Córdoba, Córdoba, Argentina

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microscope slide and coverslip with 50% glycerol in water. For TEM, grids were unmounted, washed in Milli-Q water and incubated for 5 min in 0.4% uranyl acetate and 1.8% methylcellulose. Fluorescence images were obtained using a FluoView FV 1200 microscope

Article Type:
Research Article
DOI:
https://doi.org/10.1530/ERC-18-0067
Volume/Issue:
Volume 25: Issue 10
Free access
Martin Almquist Department of Clinical Sciences Lund, Department of Surgery Section of Endocrine and Sarcoma Lund, Skåne University Hospital, Lund University, Lund, Sweden

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Elin Isaksson Department of Clinical Sciences Malmö, Urology Malmö, Faculty of Medicine, Skåne University Hospital, Lund University, Malmö, Sweden

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Naomi Clyne Department of Clinical Sciences Lund, Nephrology Lund, Faculty of Medicine, Skåne University Hospital, Lund University, Lund, Sweden

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International 2109 – 2117 . ( https://doi.org/10.1046/j.1523-1755.2001.00042.x ) Cui L Gao Y Yu H Li M Wang B Zhou T Hu Q 2017 Intraoperative parathyroid localization with near-infrared fluorescence imaging using indocyanine green during

Article Type:
Review Article
DOI:
https://doi.org/10.1530/ERC-19-0284
Volume/Issue:
Volume 27: Issue 1
Free access
Emanuel Christ Division of Endocrinology, Diabetology and Metabolism, University Hospital of Basel, University of Basel, Basel, Switzerland
Center for Neuroendocrine and Endocrine Tumors, University Hospital Basel, Basel Switzerland

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Kwadwo Antwi Clinic of Radiology and Nuclear Medicine, University Hospital, Basel, Switzerland

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Melpomeni Fani Clinic of Radiology and Nuclear Medicine, University Hospital, Basel, Switzerland

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Damian Wild Center for Neuroendocrine and Endocrine Tumors, University Hospital Basel, Basel Switzerland
Clinic of Radiology and Nuclear Medicine, University Hospital, Basel, Switzerland

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. Nuklearmedizin 58 124 . ( https://doi.org/10.1055/s-0039-1683525 ) Brand C Abdel-Atti D Zhang Y Carlin S Clardy SM Keliher EJ Weber WA Lewis JS Reiner T 2014 In vivo imaging of GLP-1R with a targeted bimodal PET/fluorescence imaging

Article Type:
Review Article
DOI:
https://doi.org/10.1530/ERC-19-0476
Volume/Issue:
Volume 27: Issue 4
Open access
Yunhui Cheng Department of Biological Chemistry, University of Michigan, Ann Arbor, MI, USA

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Raili Emilia Kerppola Atterocor Inc., Ann Arbor, MI, USA

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Tom Klaus Kerppola Department of Biological Chemistry, University of Michigan, Ann Arbor, MI, USA

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concentrations of ATR-101 for 20 h is shown. The inserts show fluorescence images of cells cultured in the presence of the indicated concentrations of ATR-101 followed by SYTOX staining. (D) Reversibility of membrane permeability and ATP depletion following ATR

Article Type:
Research Article
DOI:
https://doi.org/10.1530/ERC-15-0527
Volume/Issue:
Volume 23: Issue 4
Free access
Charlotte Nys Laboratory of Tissue Plasticity in Health and Disease, Cluster of Stem Cell and Developmental Biology, Department of Development and Regeneration, KU Leuven (University of Leuven), Leuven, Belgium

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Yu-Lun Lee Laboratory of Tissue Plasticity in Health and Disease, Cluster of Stem Cell and Developmental Biology, Department of Development and Regeneration, KU Leuven (University of Leuven), Leuven, Belgium

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Heleen Roose Laboratory of Tissue Plasticity in Health and Disease, Cluster of Stem Cell and Developmental Biology, Department of Development and Regeneration, KU Leuven (University of Leuven), Leuven, Belgium

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Freya Mertens Laboratory of Tissue Plasticity in Health and Disease, Cluster of Stem Cell and Developmental Biology, Department of Development and Regeneration, KU Leuven (University of Leuven), Leuven, Belgium
Department of Imaging and Pathology, UZ Leuven (University Hospitals Leuven), Leuven, Belgium

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Ellen De Pauw Laboratory of Tissue Plasticity in Health and Disease, Cluster of Stem Cell and Developmental Biology, Department of Development and Regeneration, KU Leuven (University of Leuven), Leuven, Belgium

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Hiroto Kobayashi Laboratory of Tissue Plasticity in Health and Disease, Cluster of Stem Cell and Developmental Biology, Department of Development and Regeneration, KU Leuven (University of Leuven), Leuven, Belgium
Department of Anatomy and Structural Science, Yamagata University Faculty of Medicine, Yamagata, Japan

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Raf Sciot Department of Imaging and Pathology, UZ Leuven (University Hospitals Leuven), Leuven, Belgium

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Marie Bex Department of Endocrinology, UZ Leuven (University Hospitals Leuven), Leuven, Belgium

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Georges Versyck Department of Neurosurgery, UZ Leuven (University Hospitals Leuven), Leuven, Belgium

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Steven De Vleeschouwer Department of Neurosurgery, UZ Leuven (University Hospitals Leuven), Leuven, Belgium

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Johannes Van Loon Department of Neurosurgery, UZ Leuven (University Hospitals Leuven), Leuven, Belgium

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Emma Laporte Laboratory of Tissue Plasticity in Health and Disease, Cluster of Stem Cell and Developmental Biology, Department of Development and Regeneration, KU Leuven (University of Leuven), Leuven, Belgium

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Hugo Vankelecom Laboratory of Tissue Plasticity in Health and Disease, Cluster of Stem Cell and Developmental Biology, Department of Development and Regeneration, KU Leuven (University of Leuven), Leuven, Belgium

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that were re-plated in Matrigel droplets, all as previously described ( Cox et al. 2019 , Vennekens et al. 2021 ; and above). Brightfield and fluorescence images were acquired using an Axiovert 40 CFL microscope (Zeiss, Jena, Germany

Article Type:
Research Article
DOI:
https://doi.org/10.1530/ERC-21-0374
Volume/Issue:
Volume 29: Issue 7
Restricted access
Erin E Swinstead Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, NIH, Bethesda, Maryland, USA

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Ville Paakinaho Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, NIH, Bethesda, Maryland, USA
Institute of Biomedicine, University of Eastern Finland, Kuopio, Finland

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Gordon L Hager Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, NIH, Bethesda, Maryland, USA

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. Live cell fluorescence imaging All the above-mentioned assays are valuable tools to characterize the action of TFs. However, they suffer from two major drawbacks; the assays average signals across populations of heterogeneous cells and rely on dead

Article Type:
Review Article
DOI:
https://doi.org/10.1530/ERC-18-0033
Volume/Issue:
Volume 25: Issue 7
Free access