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Nidal Muhanna Guided Therapeutic (GTx) Program, TECHNA Institute, Princess Margaret Cancer Center and University Health Network, Toronto, Ontario, Canada
Department of Otolaryngology-Head and Neck Surgery-Surgical Oncology, Princess Margaret Cancer Centre/University Health Network, University of Toronto, Toronto, Ontario, Canada
Department of Otolaryngology-Head and Neck Surgery, Tel Aviv Sourasky Medical Center, Tel Aviv University, Tel Aviv, Israel

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Harley H L Chan Guided Therapeutic (GTx) Program, TECHNA Institute, Princess Margaret Cancer Center and University Health Network, Toronto, Ontario, Canada
Department of Otolaryngology-Head and Neck Surgery-Surgical Oncology, Princess Margaret Cancer Centre/University Health Network, University of Toronto, Toronto, Ontario, Canada

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Jason L Townson Guided Therapeutic (GTx) Program, TECHNA Institute, Princess Margaret Cancer Center and University Health Network, Toronto, Ontario, Canada
Department of Otolaryngology-Head and Neck Surgery-Surgical Oncology, Princess Margaret Cancer Centre/University Health Network, University of Toronto, Toronto, Ontario, Canada

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Cheng S Jin Guided Therapeutic (GTx) Program, TECHNA Institute, Princess Margaret Cancer Center and University Health Network, Toronto, Ontario, Canada
Department of Otolaryngology-Head and Neck Surgery-Surgical Oncology, Princess Margaret Cancer Centre/University Health Network, University of Toronto, Toronto, Ontario, Canada

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Lili Ding Guided Therapeutic (GTx) Program, TECHNA Institute, Princess Margaret Cancer Center and University Health Network, Toronto, Ontario, Canada

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Michael S Valic Guided Therapeutic (GTx) Program, TECHNA Institute, Princess Margaret Cancer Center and University Health Network, Toronto, Ontario, Canada

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Catriona M Douglas Guided Therapeutic (GTx) Program, TECHNA Institute, Princess Margaret Cancer Center and University Health Network, Toronto, Ontario, Canada
Department of Otolaryngology-Head and Neck Surgery-Surgical Oncology, Princess Margaret Cancer Centre/University Health Network, University of Toronto, Toronto, Ontario, Canada

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Christina M MacLaughlin Guided Therapeutic (GTx) Program, TECHNA Institute, Princess Margaret Cancer Center and University Health Network, Toronto, Ontario, Canada

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Juan Chen Guided Therapeutic (GTx) Program, TECHNA Institute, Princess Margaret Cancer Center and University Health Network, Toronto, Ontario, Canada

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Gang Zheng Guided Therapeutic (GTx) Program, TECHNA Institute, Princess Margaret Cancer Center and University Health Network, Toronto, Ontario, Canada
Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada

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Jonathan C Irish Guided Therapeutic (GTx) Program, TECHNA Institute, Princess Margaret Cancer Center and University Health Network, Toronto, Ontario, Canada
Department of Otolaryngology-Head and Neck Surgery-Surgical Oncology, Princess Margaret Cancer Centre/University Health Network, University of Toronto, Toronto, Ontario, Canada

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volume of interest (VOI) was calculated. Fluorescence imaging of rabbit thyroid tumor When tumors reached 1 cm diameter at 1–2 weeks after tumor inoculation, PLP was injected intravenously through a catheter in rabbit marginal ear vein at the dose

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Gina Chia-Yi Chu Uro-Oncology Research, Surgery, Biomedical Sciences, Biostatistics and Bioinformatics Center, Department of Pathology, Department of Pathology, Department of Biochemistry and Cell Biology, Department of Medicine, Samuel Oschin Comprehensive Cancer Center, Cedars-Sinai Medical Center, 8750 Beverly Blvd., Atrium 103, Los Angeles, California 90048, USA Departments of

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Haiyen E Zhau Uro-Oncology Research, Surgery, Biomedical Sciences, Biostatistics and Bioinformatics Center, Department of Pathology, Department of Pathology, Department of Biochemistry and Cell Biology, Department of Medicine, Samuel Oschin Comprehensive Cancer Center, Cedars-Sinai Medical Center, 8750 Beverly Blvd., Atrium 103, Los Angeles, California 90048, USA Departments of

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Ruoxiang Wang Uro-Oncology Research, Surgery, Biomedical Sciences, Biostatistics and Bioinformatics Center, Department of Pathology, Department of Pathology, Department of Biochemistry and Cell Biology, Department of Medicine, Samuel Oschin Comprehensive Cancer Center, Cedars-Sinai Medical Center, 8750 Beverly Blvd., Atrium 103, Los Angeles, California 90048, USA Departments of

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André Rogatko Uro-Oncology Research, Surgery, Biomedical Sciences, Biostatistics and Bioinformatics Center, Department of Pathology, Department of Pathology, Department of Biochemistry and Cell Biology, Department of Medicine, Samuel Oschin Comprehensive Cancer Center, Cedars-Sinai Medical Center, 8750 Beverly Blvd., Atrium 103, Los Angeles, California 90048, USA Departments of
Uro-Oncology Research, Surgery, Biomedical Sciences, Biostatistics and Bioinformatics Center, Department of Pathology, Department of Pathology, Department of Biochemistry and Cell Biology, Department of Medicine, Samuel Oschin Comprehensive Cancer Center, Cedars-Sinai Medical Center, 8750 Beverly Blvd., Atrium 103, Los Angeles, California 90048, USA Departments of

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Xu Feng Uro-Oncology Research, Surgery, Biomedical Sciences, Biostatistics and Bioinformatics Center, Department of Pathology, Department of Pathology, Department of Biochemistry and Cell Biology, Department of Medicine, Samuel Oschin Comprehensive Cancer Center, Cedars-Sinai Medical Center, 8750 Beverly Blvd., Atrium 103, Los Angeles, California 90048, USA Departments of

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Majd Zayzafoon Uro-Oncology Research, Surgery, Biomedical Sciences, Biostatistics and Bioinformatics Center, Department of Pathology, Department of Pathology, Department of Biochemistry and Cell Biology, Department of Medicine, Samuel Oschin Comprehensive Cancer Center, Cedars-Sinai Medical Center, 8750 Beverly Blvd., Atrium 103, Los Angeles, California 90048, USA Departments of

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Youhua Liu Uro-Oncology Research, Surgery, Biomedical Sciences, Biostatistics and Bioinformatics Center, Department of Pathology, Department of Pathology, Department of Biochemistry and Cell Biology, Department of Medicine, Samuel Oschin Comprehensive Cancer Center, Cedars-Sinai Medical Center, 8750 Beverly Blvd., Atrium 103, Los Angeles, California 90048, USA Departments of

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Mary C Farach-Carson Uro-Oncology Research, Surgery, Biomedical Sciences, Biostatistics and Bioinformatics Center, Department of Pathology, Department of Pathology, Department of Biochemistry and Cell Biology, Department of Medicine, Samuel Oschin Comprehensive Cancer Center, Cedars-Sinai Medical Center, 8750 Beverly Blvd., Atrium 103, Los Angeles, California 90048, USA Departments of

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Sungyong You Uro-Oncology Research, Surgery, Biomedical Sciences, Biostatistics and Bioinformatics Center, Department of Pathology, Department of Pathology, Department of Biochemistry and Cell Biology, Department of Medicine, Samuel Oschin Comprehensive Cancer Center, Cedars-Sinai Medical Center, 8750 Beverly Blvd., Atrium 103, Los Angeles, California 90048, USA Departments of
Uro-Oncology Research, Surgery, Biomedical Sciences, Biostatistics and Bioinformatics Center, Department of Pathology, Department of Pathology, Department of Biochemistry and Cell Biology, Department of Medicine, Samuel Oschin Comprehensive Cancer Center, Cedars-Sinai Medical Center, 8750 Beverly Blvd., Atrium 103, Los Angeles, California 90048, USA Departments of

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Jayoung Kim Uro-Oncology Research, Surgery, Biomedical Sciences, Biostatistics and Bioinformatics Center, Department of Pathology, Department of Pathology, Department of Biochemistry and Cell Biology, Department of Medicine, Samuel Oschin Comprehensive Cancer Center, Cedars-Sinai Medical Center, 8750 Beverly Blvd., Atrium 103, Los Angeles, California 90048, USA Departments of
Uro-Oncology Research, Surgery, Biomedical Sciences, Biostatistics and Bioinformatics Center, Department of Pathology, Department of Pathology, Department of Biochemistry and Cell Biology, Department of Medicine, Samuel Oschin Comprehensive Cancer Center, Cedars-Sinai Medical Center, 8750 Beverly Blvd., Atrium 103, Los Angeles, California 90048, USA Departments of

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Michael R Freeman Uro-Oncology Research, Surgery, Biomedical Sciences, Biostatistics and Bioinformatics Center, Department of Pathology, Department of Pathology, Department of Biochemistry and Cell Biology, Department of Medicine, Samuel Oschin Comprehensive Cancer Center, Cedars-Sinai Medical Center, 8750 Beverly Blvd., Atrium 103, Los Angeles, California 90048, USA Departments of
Uro-Oncology Research, Surgery, Biomedical Sciences, Biostatistics and Bioinformatics Center, Department of Pathology, Department of Pathology, Department of Biochemistry and Cell Biology, Department of Medicine, Samuel Oschin Comprehensive Cancer Center, Cedars-Sinai Medical Center, 8750 Beverly Blvd., Atrium 103, Los Angeles, California 90048, USA Departments of
Uro-Oncology Research, Surgery, Biomedical Sciences, Biostatistics and Bioinformatics Center, Department of Pathology, Department of Pathology, Department of Biochemistry and Cell Biology, Department of Medicine, Samuel Oschin Comprehensive Cancer Center, Cedars-Sinai Medical Center, 8750 Beverly Blvd., Atrium 103, Los Angeles, California 90048, USA Departments of

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Leland W K Chung Uro-Oncology Research, Surgery, Biomedical Sciences, Biostatistics and Bioinformatics Center, Department of Pathology, Department of Pathology, Department of Biochemistry and Cell Biology, Department of Medicine, Samuel Oschin Comprehensive Cancer Center, Cedars-Sinai Medical Center, 8750 Beverly Blvd., Atrium 103, Los Angeles, California 90048, USA Departments of
Uro-Oncology Research, Surgery, Biomedical Sciences, Biostatistics and Bioinformatics Center, Department of Pathology, Department of Pathology, Department of Biochemistry and Cell Biology, Department of Medicine, Samuel Oschin Comprehensive Cancer Center, Cedars-Sinai Medical Center, 8750 Beverly Blvd., Atrium 103, Los Angeles, California 90048, USA Departments of

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–RANK signaling seems obligatory for bone colonization. LN RANKL cells with RANK knockdown ( Fig. 6 A) formed very small tumors in mice tibias, undetectable by NIR dye-based fluorescence imaging ( Yang et al . 2010 ) but detectable by X-ray ( Fig. 6 B); moreover

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Qiao Zheng Department of Cellular and Molecular Medicine, Department of Nutritional Sciences, Department of Molecular Carcinogenesis, Department of Stem Cell Biology and Regenerative Medicine, ImageIQ, Case Comprehensive Cancer Center, Lerner Research Institute, Cleveland Clinic Foundation, 9500 Euclid Avenue, NC10, Cleveland, Ohio 44195, USA

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Lauren Banaszak Department of Cellular and Molecular Medicine, Department of Nutritional Sciences, Department of Molecular Carcinogenesis, Department of Stem Cell Biology and Regenerative Medicine, ImageIQ, Case Comprehensive Cancer Center, Lerner Research Institute, Cleveland Clinic Foundation, 9500 Euclid Avenue, NC10, Cleveland, Ohio 44195, USA

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Sarah Fracci Department of Cellular and Molecular Medicine, Department of Nutritional Sciences, Department of Molecular Carcinogenesis, Department of Stem Cell Biology and Regenerative Medicine, ImageIQ, Case Comprehensive Cancer Center, Lerner Research Institute, Cleveland Clinic Foundation, 9500 Euclid Avenue, NC10, Cleveland, Ohio 44195, USA

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Diana Basali Department of Cellular and Molecular Medicine, Department of Nutritional Sciences, Department of Molecular Carcinogenesis, Department of Stem Cell Biology and Regenerative Medicine, ImageIQ, Case Comprehensive Cancer Center, Lerner Research Institute, Cleveland Clinic Foundation, 9500 Euclid Avenue, NC10, Cleveland, Ohio 44195, USA

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Sarah M Dunlap Department of Cellular and Molecular Medicine, Department of Nutritional Sciences, Department of Molecular Carcinogenesis, Department of Stem Cell Biology and Regenerative Medicine, ImageIQ, Case Comprehensive Cancer Center, Lerner Research Institute, Cleveland Clinic Foundation, 9500 Euclid Avenue, NC10, Cleveland, Ohio 44195, USA

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Stephen D Hursting Department of Cellular and Molecular Medicine, Department of Nutritional Sciences, Department of Molecular Carcinogenesis, Department of Stem Cell Biology and Regenerative Medicine, ImageIQ, Case Comprehensive Cancer Center, Lerner Research Institute, Cleveland Clinic Foundation, 9500 Euclid Avenue, NC10, Cleveland, Ohio 44195, USA
Department of Cellular and Molecular Medicine, Department of Nutritional Sciences, Department of Molecular Carcinogenesis, Department of Stem Cell Biology and Regenerative Medicine, ImageIQ, Case Comprehensive Cancer Center, Lerner Research Institute, Cleveland Clinic Foundation, 9500 Euclid Avenue, NC10, Cleveland, Ohio 44195, USA

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Jeremy N Rich Department of Cellular and Molecular Medicine, Department of Nutritional Sciences, Department of Molecular Carcinogenesis, Department of Stem Cell Biology and Regenerative Medicine, ImageIQ, Case Comprehensive Cancer Center, Lerner Research Institute, Cleveland Clinic Foundation, 9500 Euclid Avenue, NC10, Cleveland, Ohio 44195, USA
Department of Cellular and Molecular Medicine, Department of Nutritional Sciences, Department of Molecular Carcinogenesis, Department of Stem Cell Biology and Regenerative Medicine, ImageIQ, Case Comprehensive Cancer Center, Lerner Research Institute, Cleveland Clinic Foundation, 9500 Euclid Avenue, NC10, Cleveland, Ohio 44195, USA

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Anita B Hjlemeland Department of Cellular and Molecular Medicine, Department of Nutritional Sciences, Department of Molecular Carcinogenesis, Department of Stem Cell Biology and Regenerative Medicine, ImageIQ, Case Comprehensive Cancer Center, Lerner Research Institute, Cleveland Clinic Foundation, 9500 Euclid Avenue, NC10, Cleveland, Ohio 44195, USA
Department of Cellular and Molecular Medicine, Department of Nutritional Sciences, Department of Molecular Carcinogenesis, Department of Stem Cell Biology and Regenerative Medicine, ImageIQ, Case Comprehensive Cancer Center, Lerner Research Institute, Cleveland Clinic Foundation, 9500 Euclid Avenue, NC10, Cleveland, Ohio 44195, USA

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Amit Vasanji Department of Cellular and Molecular Medicine, Department of Nutritional Sciences, Department of Molecular Carcinogenesis, Department of Stem Cell Biology and Regenerative Medicine, ImageIQ, Case Comprehensive Cancer Center, Lerner Research Institute, Cleveland Clinic Foundation, 9500 Euclid Avenue, NC10, Cleveland, Ohio 44195, USA

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Nathan A Berger Department of Cellular and Molecular Medicine, Department of Nutritional Sciences, Department of Molecular Carcinogenesis, Department of Stem Cell Biology and Regenerative Medicine, ImageIQ, Case Comprehensive Cancer Center, Lerner Research Institute, Cleveland Clinic Foundation, 9500 Euclid Avenue, NC10, Cleveland, Ohio 44195, USA

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Justin D Lathia Department of Cellular and Molecular Medicine, Department of Nutritional Sciences, Department of Molecular Carcinogenesis, Department of Stem Cell Biology and Regenerative Medicine, ImageIQ, Case Comprehensive Cancer Center, Lerner Research Institute, Cleveland Clinic Foundation, 9500 Euclid Avenue, NC10, Cleveland, Ohio 44195, USA
Department of Cellular and Molecular Medicine, Department of Nutritional Sciences, Department of Molecular Carcinogenesis, Department of Stem Cell Biology and Regenerative Medicine, ImageIQ, Case Comprehensive Cancer Center, Lerner Research Institute, Cleveland Clinic Foundation, 9500 Euclid Avenue, NC10, Cleveland, Ohio 44195, USA

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Ofer Reizes Department of Cellular and Molecular Medicine, Department of Nutritional Sciences, Department of Molecular Carcinogenesis, Department of Stem Cell Biology and Regenerative Medicine, ImageIQ, Case Comprehensive Cancer Center, Lerner Research Institute, Cleveland Clinic Foundation, 9500 Euclid Avenue, NC10, Cleveland, Ohio 44195, USA
Department of Cellular and Molecular Medicine, Department of Nutritional Sciences, Department of Molecular Carcinogenesis, Department of Stem Cell Biology and Regenerative Medicine, ImageIQ, Case Comprehensive Cancer Center, Lerner Research Institute, Cleveland Clinic Foundation, 9500 Euclid Avenue, NC10, Cleveland, Ohio 44195, USA

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. Lineage tracing analysis Lineage tracing was performed as previously described ( Lathia et al . 2011 ). Briefly, phase-contrast, time-lapse image stacks consisting of 288 frames (5 min intervals) and corresponding fluorescence image stacks (72 frames, 20

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Martin Ullrich Department of Radiopharmaceutical and Chemical Biology, Helmholtz-Zentrum Dresden-Rossendorf, Institute of Radiopharmaceutical Cancer Research, Dresden, Germany

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Josephine Liers Department of Radiopharmaceutical and Chemical Biology, Helmholtz-Zentrum Dresden-Rossendorf, Institute of Radiopharmaceutical Cancer Research, Dresden, Germany
Technische Universität Dresden, School of Medicine, Faculty of Medicine Carl Gustav Carus, Dresden, Germany

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Mirko Peitzsch Technische Universität Dresden, University Hospital Carl Gustav Carus, Institute of Clinical Chemistry and Laboratory Medicine, Dresden, Germany

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Anja Feldmann Department of Radioimmunology, Helmholtz-Zentrum Dresden-Rossendorf, Institute of Radiopharmaceutical Cancer Research, Dresden, Germany

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Ralf Bergmann Department of Radiopharmaceutical and Chemical Biology, Helmholtz-Zentrum Dresden-Rossendorf, Institute of Radiopharmaceutical Cancer Research, Dresden, Germany

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Ulrich Sommer Technische Universität Dresden, University Hospital Carl Gustav Carus, Institute of Pathology, Dresden, Germany

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Susan Richter Technische Universität Dresden, School of Medicine, Faculty of Medicine Carl Gustav Carus, Dresden, Germany
Technische Universität Dresden, University Hospital Carl Gustav Carus, Institute of Clinical Chemistry and Laboratory Medicine, Dresden, Germany

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Stefan R Bornstein Technische Universität Dresden, School of Medicine, Faculty of Medicine Carl Gustav Carus, Dresden, Germany
Department of Internal Medicine III, Technische Universität Dresden, University Hospital Carl Gustav Carus, Dresden, Germany

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Michael Bachmann Technische Universität Dresden, School of Medicine, Faculty of Medicine Carl Gustav Carus, Dresden, Germany
Department of Radioimmunology, Helmholtz-Zentrum Dresden-Rossendorf, Institute of Radiopharmaceutical Cancer Research, Dresden, Germany
Technische Universität Dresden, University Hospital Carl Gustav Carus, Universitäts Krebs Centrum (UCC), Tumorimmunology, Dresden, Germany
Technische Universität Dresden, National Center for Tumor Diseases (NCT), Dresden, Germany

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Graeme Eisenhofer Technische Universität Dresden, School of Medicine, Faculty of Medicine Carl Gustav Carus, Dresden, Germany
Technische Universität Dresden, University Hospital Carl Gustav Carus, Institute of Clinical Chemistry and Laboratory Medicine, Dresden, Germany
Department of Internal Medicine III, Technische Universität Dresden, University Hospital Carl Gustav Carus, Dresden, Germany

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Christian G Ziegler Department of Internal Medicine III, Technische Universität Dresden, University Hospital Carl Gustav Carus, Dresden, Germany

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Jens Pietzsch Department of Radiopharmaceutical and Chemical Biology, Helmholtz-Zentrum Dresden-Rossendorf, Institute of Radiopharmaceutical Cancer Research, Dresden, Germany
Technische Universität Dresden, School of Science, Faculty of Chemistry and Food Chemistry, Dresden, Germany

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). This particular model resembles at least in part biochemical features and molecular characteristics of sporadic and hereditary human PPGLs and allows for monitoring tumor progression and treatment response using both in vivo fluorescence imaging and

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Carla S Verissimo
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Jan J Molenaar Division of Medical Pharmacology, Department of Human Genetics, Division of Toxicology, Leiden/Amsterdam Center for Drug Research, Gorlaeus Laboratories, Leiden, The Netherlands

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John Meerman Division of Medical Pharmacology, Department of Human Genetics, Division of Toxicology, Leiden/Amsterdam Center for Drug Research, Gorlaeus Laboratories, Leiden, The Netherlands

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Jordi Carreras Puigvert Division of Medical Pharmacology, Department of Human Genetics, Division of Toxicology, Leiden/Amsterdam Center for Drug Research, Gorlaeus Laboratories, Leiden, The Netherlands

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Fieke Lamers Division of Medical Pharmacology, Department of Human Genetics, Division of Toxicology, Leiden/Amsterdam Center for Drug Research, Gorlaeus Laboratories, Leiden, The Netherlands

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Jan Koster Division of Medical Pharmacology, Department of Human Genetics, Division of Toxicology, Leiden/Amsterdam Center for Drug Research, Gorlaeus Laboratories, Leiden, The Netherlands

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Erik H J Danen Division of Medical Pharmacology, Department of Human Genetics, Division of Toxicology, Leiden/Amsterdam Center for Drug Research, Gorlaeus Laboratories, Leiden, The Netherlands

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Bob van de Water Division of Medical Pharmacology, Department of Human Genetics, Division of Toxicology, Leiden/Amsterdam Center for Drug Research, Gorlaeus Laboratories, Leiden, The Netherlands

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Rogier Versteeg Division of Medical Pharmacology, Department of Human Genetics, Division of Toxicology, Leiden/Amsterdam Center for Drug Research, Gorlaeus Laboratories, Leiden, The Netherlands

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Carlos P Fitzsimons
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Erno Vreugdenhil
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interference contrast (DIC) and fluorescence imaging were performed on a Nikon TE-2000 E system under 37 °C and 5% CO 2 controlled conditions. Time-lapse imaging of phosphatidylserine translocation Forty-eight hours after N1E-115 cells had been transfected

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Xin Qiu Department of Obstetrics and Gynaecology Child and Family Research Institute, University of British Columbia, Vancouver, British Columbia, Canada V5Z 4H4

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Jung-Chien Cheng Department of Obstetrics and Gynaecology Child and Family Research Institute, University of British Columbia, Vancouver, British Columbia, Canada V5Z 4H4

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Hsun-Ming Chang Department of Obstetrics and Gynaecology Child and Family Research Institute, University of British Columbia, Vancouver, British Columbia, Canada V5Z 4H4

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Peter C K Leung Department of Obstetrics and Gynaecology Child and Family Research Institute, University of British Columbia, Vancouver, British Columbia, Canada V5Z 4H4

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. Non-invading cells were removed from the upper side of the filter, and the nuclei of the invasive cells were stained with Hoechst 33258. The top panel shows representative fluorescence images obtained in the invasion assays. The scale bar represents

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Françoise Galland Assistance Publique-Hôpitaux de Paris, Institut National de la Santé et de la Recherche Médicale, Institut de Cancérologie Gustave-Roussy, Centre National de la Recherche Scientifique, Département de Génomique Fonctionnelle, Assistance Publique-Hôpitaux de Paris, Hôpital Foch, Service de Neurochirurgie, Institut National de la Santé et de la Recherche Médicale, Université Paris-Sud 11, Hôpital de Bicêtre, Service d'Endocrinologie et des Maladies de la Reproduction, Le Kremlin-Bicêtre F-94275, France
Assistance Publique-Hôpitaux de Paris, Institut National de la Santé et de la Recherche Médicale, Institut de Cancérologie Gustave-Roussy, Centre National de la Recherche Scientifique, Département de Génomique Fonctionnelle, Assistance Publique-Hôpitaux de Paris, Hôpital Foch, Service de Neurochirurgie, Institut National de la Santé et de la Recherche Médicale, Université Paris-Sud 11, Hôpital de Bicêtre, Service d'Endocrinologie et des Maladies de la Reproduction, Le Kremlin-Bicêtre F-94275, France

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Ludovic Lacroix Assistance Publique-Hôpitaux de Paris, Institut National de la Santé et de la Recherche Médicale, Institut de Cancérologie Gustave-Roussy, Centre National de la Recherche Scientifique, Département de Génomique Fonctionnelle, Assistance Publique-Hôpitaux de Paris, Hôpital Foch, Service de Neurochirurgie, Institut National de la Santé et de la Recherche Médicale, Université Paris-Sud 11, Hôpital de Bicêtre, Service d'Endocrinologie et des Maladies de la Reproduction, Le Kremlin-Bicêtre F-94275, France
Assistance Publique-Hôpitaux de Paris, Institut National de la Santé et de la Recherche Médicale, Institut de Cancérologie Gustave-Roussy, Centre National de la Recherche Scientifique, Département de Génomique Fonctionnelle, Assistance Publique-Hôpitaux de Paris, Hôpital Foch, Service de Neurochirurgie, Institut National de la Santé et de la Recherche Médicale, Université Paris-Sud 11, Hôpital de Bicêtre, Service d'Endocrinologie et des Maladies de la Reproduction, Le Kremlin-Bicêtre F-94275, France

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Patrick Saulnier Assistance Publique-Hôpitaux de Paris, Institut National de la Santé et de la Recherche Médicale, Institut de Cancérologie Gustave-Roussy, Centre National de la Recherche Scientifique, Département de Génomique Fonctionnelle, Assistance Publique-Hôpitaux de Paris, Hôpital Foch, Service de Neurochirurgie, Institut National de la Santé et de la Recherche Médicale, Université Paris-Sud 11, Hôpital de Bicêtre, Service d'Endocrinologie et des Maladies de la Reproduction, Le Kremlin-Bicêtre F-94275, France

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Philippe Dessen Assistance Publique-Hôpitaux de Paris, Institut National de la Santé et de la Recherche Médicale, Institut de Cancérologie Gustave-Roussy, Centre National de la Recherche Scientifique, Département de Génomique Fonctionnelle, Assistance Publique-Hôpitaux de Paris, Hôpital Foch, Service de Neurochirurgie, Institut National de la Santé et de la Recherche Médicale, Université Paris-Sud 11, Hôpital de Bicêtre, Service d'Endocrinologie et des Maladies de la Reproduction, Le Kremlin-Bicêtre F-94275, France
Assistance Publique-Hôpitaux de Paris, Institut National de la Santé et de la Recherche Médicale, Institut de Cancérologie Gustave-Roussy, Centre National de la Recherche Scientifique, Département de Génomique Fonctionnelle, Assistance Publique-Hôpitaux de Paris, Hôpital Foch, Service de Neurochirurgie, Institut National de la Santé et de la Recherche Médicale, Université Paris-Sud 11, Hôpital de Bicêtre, Service d'Endocrinologie et des Maladies de la Reproduction, Le Kremlin-Bicêtre F-94275, France

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Geri Meduri Assistance Publique-Hôpitaux de Paris, Institut National de la Santé et de la Recherche Médicale, Institut de Cancérologie Gustave-Roussy, Centre National de la Recherche Scientifique, Département de Génomique Fonctionnelle, Assistance Publique-Hôpitaux de Paris, Hôpital Foch, Service de Neurochirurgie, Institut National de la Santé et de la Recherche Médicale, Université Paris-Sud 11, Hôpital de Bicêtre, Service d'Endocrinologie et des Maladies de la Reproduction, Le Kremlin-Bicêtre F-94275, France
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Assistance Publique-Hôpitaux de Paris, Institut National de la Santé et de la Recherche Médicale, Institut de Cancérologie Gustave-Roussy, Centre National de la Recherche Scientifique, Département de Génomique Fonctionnelle, Assistance Publique-Hôpitaux de Paris, Hôpital Foch, Service de Neurochirurgie, Institut National de la Santé et de la Recherche Médicale, Université Paris-Sud 11, Hôpital de Bicêtre, Service d'Endocrinologie et des Maladies de la Reproduction, Le Kremlin-Bicêtre F-94275, France

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Michèle Bernier Assistance Publique-Hôpitaux de Paris, Institut National de la Santé et de la Recherche Médicale, Institut de Cancérologie Gustave-Roussy, Centre National de la Recherche Scientifique, Département de Génomique Fonctionnelle, Assistance Publique-Hôpitaux de Paris, Hôpital Foch, Service de Neurochirurgie, Institut National de la Santé et de la Recherche Médicale, Université Paris-Sud 11, Hôpital de Bicêtre, Service d'Endocrinologie et des Maladies de la Reproduction, Le Kremlin-Bicêtre F-94275, France

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Stéphane Gaillard Assistance Publique-Hôpitaux de Paris, Institut National de la Santé et de la Recherche Médicale, Institut de Cancérologie Gustave-Roussy, Centre National de la Recherche Scientifique, Département de Génomique Fonctionnelle, Assistance Publique-Hôpitaux de Paris, Hôpital Foch, Service de Neurochirurgie, Institut National de la Santé et de la Recherche Médicale, Université Paris-Sud 11, Hôpital de Bicêtre, Service d'Endocrinologie et des Maladies de la Reproduction, Le Kremlin-Bicêtre F-94275, France

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Jean Guibourdenche Assistance Publique-Hôpitaux de Paris, Institut National de la Santé et de la Recherche Médicale, Institut de Cancérologie Gustave-Roussy, Centre National de la Recherche Scientifique, Département de Génomique Fonctionnelle, Assistance Publique-Hôpitaux de Paris, Hôpital Foch, Service de Neurochirurgie, Institut National de la Santé et de la Recherche Médicale, Université Paris-Sud 11, Hôpital de Bicêtre, Service d'Endocrinologie et des Maladies de la Reproduction, Le Kremlin-Bicêtre F-94275, France
Assistance Publique-Hôpitaux de Paris, Institut National de la Santé et de la Recherche Médicale, Institut de Cancérologie Gustave-Roussy, Centre National de la Recherche Scientifique, Département de Génomique Fonctionnelle, Assistance Publique-Hôpitaux de Paris, Hôpital Foch, Service de Neurochirurgie, Institut National de la Santé et de la Recherche Médicale, Université Paris-Sud 11, Hôpital de Bicêtre, Service d'Endocrinologie et des Maladies de la Reproduction, Le Kremlin-Bicêtre F-94275, France

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Thierry Fournier Assistance Publique-Hôpitaux de Paris, Institut National de la Santé et de la Recherche Médicale, Institut de Cancérologie Gustave-Roussy, Centre National de la Recherche Scientifique, Département de Génomique Fonctionnelle, Assistance Publique-Hôpitaux de Paris, Hôpital Foch, Service de Neurochirurgie, Institut National de la Santé et de la Recherche Médicale, Université Paris-Sud 11, Hôpital de Bicêtre, Service d'Endocrinologie et des Maladies de la Reproduction, Le Kremlin-Bicêtre F-94275, France

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Danièle Evain-Brion Assistance Publique-Hôpitaux de Paris, Institut National de la Santé et de la Recherche Médicale, Institut de Cancérologie Gustave-Roussy, Centre National de la Recherche Scientifique, Département de Génomique Fonctionnelle, Assistance Publique-Hôpitaux de Paris, Hôpital Foch, Service de Neurochirurgie, Institut National de la Santé et de la Recherche Médicale, Université Paris-Sud 11, Hôpital de Bicêtre, Service d'Endocrinologie et des Maladies de la Reproduction, Le Kremlin-Bicêtre F-94275, France
Assistance Publique-Hôpitaux de Paris, Institut National de la Santé et de la Recherche Médicale, Institut de Cancérologie Gustave-Roussy, Centre National de la Recherche Scientifique, Département de Génomique Fonctionnelle, Assistance Publique-Hôpitaux de Paris, Hôpital Foch, Service de Neurochirurgie, Institut National de la Santé et de la Recherche Médicale, Université Paris-Sud 11, Hôpital de Bicêtre, Service d'Endocrinologie et des Maladies de la Reproduction, Le Kremlin-Bicêtre F-94275, France

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Jean Michel Bidart Assistance Publique-Hôpitaux de Paris, Institut National de la Santé et de la Recherche Médicale, Institut de Cancérologie Gustave-Roussy, Centre National de la Recherche Scientifique, Département de Génomique Fonctionnelle, Assistance Publique-Hôpitaux de Paris, Hôpital Foch, Service de Neurochirurgie, Institut National de la Santé et de la Recherche Médicale, Université Paris-Sud 11, Hôpital de Bicêtre, Service d'Endocrinologie et des Maladies de la Reproduction, Le Kremlin-Bicêtre F-94275, France
Assistance Publique-Hôpitaux de Paris, Institut National de la Santé et de la Recherche Médicale, Institut de Cancérologie Gustave-Roussy, Centre National de la Recherche Scientifique, Département de Génomique Fonctionnelle, Assistance Publique-Hôpitaux de Paris, Hôpital Foch, Service de Neurochirurgie, Institut National de la Santé et de la Recherche Médicale, Université Paris-Sud 11, Hôpital de Bicêtre, Service d'Endocrinologie et des Maladies de la Reproduction, Le Kremlin-Bicêtre F-94275, France

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Philippe Chanson Assistance Publique-Hôpitaux de Paris, Institut National de la Santé et de la Recherche Médicale, Institut de Cancérologie Gustave-Roussy, Centre National de la Recherche Scientifique, Département de Génomique Fonctionnelle, Assistance Publique-Hôpitaux de Paris, Hôpital Foch, Service de Neurochirurgie, Institut National de la Santé et de la Recherche Médicale, Université Paris-Sud 11, Hôpital de Bicêtre, Service d'Endocrinologie et des Maladies de la Reproduction, Le Kremlin-Bicêtre F-94275, France
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Assistance Publique-Hôpitaux de Paris, Institut National de la Santé et de la Recherche Médicale, Institut de Cancérologie Gustave-Roussy, Centre National de la Recherche Scientifique, Département de Génomique Fonctionnelle, Assistance Publique-Hôpitaux de Paris, Hôpital Foch, Service de Neurochirurgie, Institut National de la Santé et de la Recherche Médicale, Université Paris-Sud 11, Hôpital de Bicêtre, Service d'Endocrinologie et des Maladies de la Reproduction, Le Kremlin-Bicêtre F-94275, France

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cRNA from the reference pool. The arrays were then washed with 0.6× and 0.01× SSC buffers containing Triton, and were dried with a nitrogen gun before scanning with an Agilent DNA microarray scanner. The fluorescence images thus obtained were quantified

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V Berno
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(red) in breast carcinoma cells grown on glass coverslips coated with LN-1 (A) or pG-LN-1 (B) for 1 h. Panel C shows higher magnified image of filopodia (red boxed area in panel B). Overlay of individual fluorescence images was carried out using

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Samah Rafehi Translational Ovarian Cancer Research Program, Department of Anatomy and Cell Biology, Department of Biochemistry, Department of Obstetrics and Gynaecology, Department of Oncology, London Regional Cancer Program, 790 Commissioners Road East, Room A4-836, London, Ontario, Canada N6A 4L6
Translational Ovarian Cancer Research Program, Department of Anatomy and Cell Biology, Department of Biochemistry, Department of Obstetrics and Gynaecology, Department of Oncology, London Regional Cancer Program, 790 Commissioners Road East, Room A4-836, London, Ontario, Canada N6A 4L6

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Yudith Ramos Valdes Translational Ovarian Cancer Research Program, Department of Anatomy and Cell Biology, Department of Biochemistry, Department of Obstetrics and Gynaecology, Department of Oncology, London Regional Cancer Program, 790 Commissioners Road East, Room A4-836, London, Ontario, Canada N6A 4L6

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Monique Bertrand Translational Ovarian Cancer Research Program, Department of Anatomy and Cell Biology, Department of Biochemistry, Department of Obstetrics and Gynaecology, Department of Oncology, London Regional Cancer Program, 790 Commissioners Road East, Room A4-836, London, Ontario, Canada N6A 4L6
Translational Ovarian Cancer Research Program, Department of Anatomy and Cell Biology, Department of Biochemistry, Department of Obstetrics and Gynaecology, Department of Oncology, London Regional Cancer Program, 790 Commissioners Road East, Room A4-836, London, Ontario, Canada N6A 4L6
Translational Ovarian Cancer Research Program, Department of Anatomy and Cell Biology, Department of Biochemistry, Department of Obstetrics and Gynaecology, Department of Oncology, London Regional Cancer Program, 790 Commissioners Road East, Room A4-836, London, Ontario, Canada N6A 4L6

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Jacob McGee Translational Ovarian Cancer Research Program, Department of Anatomy and Cell Biology, Department of Biochemistry, Department of Obstetrics and Gynaecology, Department of Oncology, London Regional Cancer Program, 790 Commissioners Road East, Room A4-836, London, Ontario, Canada N6A 4L6
Translational Ovarian Cancer Research Program, Department of Anatomy and Cell Biology, Department of Biochemistry, Department of Obstetrics and Gynaecology, Department of Oncology, London Regional Cancer Program, 790 Commissioners Road East, Room A4-836, London, Ontario, Canada N6A 4L6

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Michel Préfontaine Translational Ovarian Cancer Research Program, Department of Anatomy and Cell Biology, Department of Biochemistry, Department of Obstetrics and Gynaecology, Department of Oncology, London Regional Cancer Program, 790 Commissioners Road East, Room A4-836, London, Ontario, Canada N6A 4L6
Translational Ovarian Cancer Research Program, Department of Anatomy and Cell Biology, Department of Biochemistry, Department of Obstetrics and Gynaecology, Department of Oncology, London Regional Cancer Program, 790 Commissioners Road East, Room A4-836, London, Ontario, Canada N6A 4L6

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Akira Sugimoto Translational Ovarian Cancer Research Program, Department of Anatomy and Cell Biology, Department of Biochemistry, Department of Obstetrics and Gynaecology, Department of Oncology, London Regional Cancer Program, 790 Commissioners Road East, Room A4-836, London, Ontario, Canada N6A 4L6
Translational Ovarian Cancer Research Program, Department of Anatomy and Cell Biology, Department of Biochemistry, Department of Obstetrics and Gynaecology, Department of Oncology, London Regional Cancer Program, 790 Commissioners Road East, Room A4-836, London, Ontario, Canada N6A 4L6
Translational Ovarian Cancer Research Program, Department of Anatomy and Cell Biology, Department of Biochemistry, Department of Obstetrics and Gynaecology, Department of Oncology, London Regional Cancer Program, 790 Commissioners Road East, Room A4-836, London, Ontario, Canada N6A 4L6

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Gabriel E DiMattia Translational Ovarian Cancer Research Program, Department of Anatomy and Cell Biology, Department of Biochemistry, Department of Obstetrics and Gynaecology, Department of Oncology, London Regional Cancer Program, 790 Commissioners Road East, Room A4-836, London, Ontario, Canada N6A 4L6
Translational Ovarian Cancer Research Program, Department of Anatomy and Cell Biology, Department of Biochemistry, Department of Obstetrics and Gynaecology, Department of Oncology, London Regional Cancer Program, 790 Commissioners Road East, Room A4-836, London, Ontario, Canada N6A 4L6
Translational Ovarian Cancer Research Program, Department of Anatomy and Cell Biology, Department of Biochemistry, Department of Obstetrics and Gynaecology, Department of Oncology, London Regional Cancer Program, 790 Commissioners Road East, Room A4-836, London, Ontario, Canada N6A 4L6
Translational Ovarian Cancer Research Program, Department of Anatomy and Cell Biology, Department of Biochemistry, Department of Obstetrics and Gynaecology, Department of Oncology, London Regional Cancer Program, 790 Commissioners Road East, Room A4-836, London, Ontario, Canada N6A 4L6

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Trevor G Shepherd Translational Ovarian Cancer Research Program, Department of Anatomy and Cell Biology, Department of Biochemistry, Department of Obstetrics and Gynaecology, Department of Oncology, London Regional Cancer Program, 790 Commissioners Road East, Room A4-836, London, Ontario, Canada N6A 4L6
Translational Ovarian Cancer Research Program, Department of Anatomy and Cell Biology, Department of Biochemistry, Department of Obstetrics and Gynaecology, Department of Oncology, London Regional Cancer Program, 790 Commissioners Road East, Room A4-836, London, Ontario, Canada N6A 4L6
Translational Ovarian Cancer Research Program, Department of Anatomy and Cell Biology, Department of Biochemistry, Department of Obstetrics and Gynaecology, Department of Oncology, London Regional Cancer Program, 790 Commissioners Road East, Room A4-836, London, Ontario, Canada N6A 4L6
Translational Ovarian Cancer Research Program, Department of Anatomy and Cell Biology, Department of Biochemistry, Department of Obstetrics and Gynaecology, Department of Oncology, London Regional Cancer Program, 790 Commissioners Road East, Room A4-836, London, Ontario, Canada N6A 4L6

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Fluor 568-conjugated phalloidin (A12380, Invitrogen). Coverslips were mounted in VectaShield mounting medium (Vector Laboratories) containing 4′,6-diamidino-2-phenylindole (1:1000; Sigma). Fluorescence images of mounted coverslips were captured using a

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Birgit Luef Division of Experimental Urology, Department of Urology, Medical University of Innsbruck, Innsbruck, Austria

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Florian Handle Division of Experimental Urology, Department of Urology, Medical University of Innsbruck, Innsbruck, Austria

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Gvantsa Kharaishvili Department of Clinical and Molecular Pathology and Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic

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Martina Hager Department of Pathology, Paracelsus Medical University, Salzburg, Austria

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Johannes Rainer Division of Molecular Pathophysiology, Biocenter, Medical University of Innsbruck, Innsbruck, Austria

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Günter Janetschek Department of Urology, Paracelsus Medical University, Salzburg, Austria

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Stephan Hruby Department of Urology, Paracelsus Medical University, Salzburg, Austria

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Christine Englberger Department of Urology, Paracelsus Medical University, Salzburg, Austria

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Jan Bouchal Department of Clinical and Molecular Pathology and Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic

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Frédéric R Santer Division of Experimental Urology, Department of Urology, Medical University of Innsbruck, Innsbruck, Austria

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Zoran Culig Division of Experimental Urology, Department of Urology, Medical University of Innsbruck, Innsbruck, Austria

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:10,000) and fluorescence images of the lower FluoroBlok membrane were taken using an Olympus IX70 microscope with a 4× objective. Determination of the number of migrated or invaded cells was performed using ImageJ software and normalized to the number of cells

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