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Department of Otolaryngology-Head and Neck Surgery-Surgical Oncology, Princess Margaret Cancer Centre/University Health Network, University of Toronto, Toronto, Ontario, Canada
Department of Otolaryngology-Head and Neck Surgery, Tel Aviv Sourasky Medical Center, Tel Aviv University, Tel Aviv, Israel
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Department of Otolaryngology-Head and Neck Surgery-Surgical Oncology, Princess Margaret Cancer Centre/University Health Network, University of Toronto, Toronto, Ontario, Canada
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Department of Otolaryngology-Head and Neck Surgery-Surgical Oncology, Princess Margaret Cancer Centre/University Health Network, University of Toronto, Toronto, Ontario, Canada
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Department of Otolaryngology-Head and Neck Surgery-Surgical Oncology, Princess Margaret Cancer Centre/University Health Network, University of Toronto, Toronto, Ontario, Canada
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Department of Otolaryngology-Head and Neck Surgery-Surgical Oncology, Princess Margaret Cancer Centre/University Health Network, University of Toronto, Toronto, Ontario, Canada
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Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada
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Department of Otolaryngology-Head and Neck Surgery-Surgical Oncology, Princess Margaret Cancer Centre/University Health Network, University of Toronto, Toronto, Ontario, Canada
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volume of interest (VOI) was calculated. Fluorescence imaging of rabbit thyroid tumor When tumors reached 1 cm diameter at 1–2 weeks after tumor inoculation, PLP was injected intravenously through a catheter in rabbit marginal ear vein at the dose
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Uro-Oncology Research, Surgery, Biomedical Sciences, Biostatistics and Bioinformatics Center, Department of Pathology, Department of Pathology, Department of Biochemistry and Cell Biology, Department of Medicine, Samuel Oschin Comprehensive Cancer Center, Cedars-Sinai Medical Center, 8750 Beverly Blvd., Atrium 103, Los Angeles, California 90048, USA Departments of
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Uro-Oncology Research, Surgery, Biomedical Sciences, Biostatistics and Bioinformatics Center, Department of Pathology, Department of Pathology, Department of Biochemistry and Cell Biology, Department of Medicine, Samuel Oschin Comprehensive Cancer Center, Cedars-Sinai Medical Center, 8750 Beverly Blvd., Atrium 103, Los Angeles, California 90048, USA Departments of
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Uro-Oncology Research, Surgery, Biomedical Sciences, Biostatistics and Bioinformatics Center, Department of Pathology, Department of Pathology, Department of Biochemistry and Cell Biology, Department of Medicine, Samuel Oschin Comprehensive Cancer Center, Cedars-Sinai Medical Center, 8750 Beverly Blvd., Atrium 103, Los Angeles, California 90048, USA Departments of
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Uro-Oncology Research, Surgery, Biomedical Sciences, Biostatistics and Bioinformatics Center, Department of Pathology, Department of Pathology, Department of Biochemistry and Cell Biology, Department of Medicine, Samuel Oschin Comprehensive Cancer Center, Cedars-Sinai Medical Center, 8750 Beverly Blvd., Atrium 103, Los Angeles, California 90048, USA Departments of
Uro-Oncology Research, Surgery, Biomedical Sciences, Biostatistics and Bioinformatics Center, Department of Pathology, Department of Pathology, Department of Biochemistry and Cell Biology, Department of Medicine, Samuel Oschin Comprehensive Cancer Center, Cedars-Sinai Medical Center, 8750 Beverly Blvd., Atrium 103, Los Angeles, California 90048, USA Departments of
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Uro-Oncology Research, Surgery, Biomedical Sciences, Biostatistics and Bioinformatics Center, Department of Pathology, Department of Pathology, Department of Biochemistry and Cell Biology, Department of Medicine, Samuel Oschin Comprehensive Cancer Center, Cedars-Sinai Medical Center, 8750 Beverly Blvd., Atrium 103, Los Angeles, California 90048, USA Departments of
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–RANK signaling seems obligatory for bone colonization. LN RANKL cells with RANK knockdown ( Fig. 6 A) formed very small tumors in mice tibias, undetectable by NIR dye-based fluorescence imaging ( Yang et al . 2010 ) but detectable by X-ray ( Fig. 6 B); moreover
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Department of Cellular and Molecular Medicine, Department of Nutritional Sciences, Department of Molecular Carcinogenesis, Department of Stem Cell Biology and Regenerative Medicine, ImageIQ, Case Comprehensive Cancer Center, Lerner Research Institute, Cleveland Clinic Foundation, 9500 Euclid Avenue, NC10, Cleveland, Ohio 44195, USA
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Department of Cellular and Molecular Medicine, Department of Nutritional Sciences, Department of Molecular Carcinogenesis, Department of Stem Cell Biology and Regenerative Medicine, ImageIQ, Case Comprehensive Cancer Center, Lerner Research Institute, Cleveland Clinic Foundation, 9500 Euclid Avenue, NC10, Cleveland, Ohio 44195, USA
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Department of Cellular and Molecular Medicine, Department of Nutritional Sciences, Department of Molecular Carcinogenesis, Department of Stem Cell Biology and Regenerative Medicine, ImageIQ, Case Comprehensive Cancer Center, Lerner Research Institute, Cleveland Clinic Foundation, 9500 Euclid Avenue, NC10, Cleveland, Ohio 44195, USA
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Department of Cellular and Molecular Medicine, Department of Nutritional Sciences, Department of Molecular Carcinogenesis, Department of Stem Cell Biology and Regenerative Medicine, ImageIQ, Case Comprehensive Cancer Center, Lerner Research Institute, Cleveland Clinic Foundation, 9500 Euclid Avenue, NC10, Cleveland, Ohio 44195, USA
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Department of Cellular and Molecular Medicine, Department of Nutritional Sciences, Department of Molecular Carcinogenesis, Department of Stem Cell Biology and Regenerative Medicine, ImageIQ, Case Comprehensive Cancer Center, Lerner Research Institute, Cleveland Clinic Foundation, 9500 Euclid Avenue, NC10, Cleveland, Ohio 44195, USA
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. Lineage tracing analysis Lineage tracing was performed as previously described ( Lathia et al . 2011 ). Briefly, phase-contrast, time-lapse image stacks consisting of 288 frames (5 min intervals) and corresponding fluorescence image stacks (72 frames, 20
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Technische Universität Dresden, School of Medicine, Faculty of Medicine Carl Gustav Carus, Dresden, Germany
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Technische Universität Dresden, University Hospital Carl Gustav Carus, Institute of Clinical Chemistry and Laboratory Medicine, Dresden, Germany
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Department of Internal Medicine III, Technische Universität Dresden, University Hospital Carl Gustav Carus, Dresden, Germany
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Department of Radioimmunology, Helmholtz-Zentrum Dresden-Rossendorf, Institute of Radiopharmaceutical Cancer Research, Dresden, Germany
Technische Universität Dresden, University Hospital Carl Gustav Carus, Universitäts Krebs Centrum (UCC), Tumorimmunology, Dresden, Germany
Technische Universität Dresden, National Center for Tumor Diseases (NCT), Dresden, Germany
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Technische Universität Dresden, University Hospital Carl Gustav Carus, Institute of Clinical Chemistry and Laboratory Medicine, Dresden, Germany
Department of Internal Medicine III, Technische Universität Dresden, University Hospital Carl Gustav Carus, Dresden, Germany
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Technische Universität Dresden, School of Science, Faculty of Chemistry and Food Chemistry, Dresden, Germany
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). This particular model resembles at least in part biochemical features and molecular characteristics of sporadic and hereditary human PPGLs and allows for monitoring tumor progression and treatment response using both in vivo fluorescence imaging and
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interference contrast (DIC) and fluorescence imaging were performed on a Nikon TE-2000 E system under 37 °C and 5% CO 2 controlled conditions. Time-lapse imaging of phosphatidylserine translocation Forty-eight hours after N1E-115 cells had been transfected
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. Non-invading cells were removed from the upper side of the filter, and the nuclei of the invasive cells were stained with Hoechst 33258. The top panel shows representative fluorescence images obtained in the invasion assays. The scale bar represents
Assistance Publique-Hôpitaux de Paris, Institut National de la Santé et de la Recherche Médicale, Institut de Cancérologie Gustave-Roussy, Centre National de la Recherche Scientifique, Département de Génomique Fonctionnelle, Assistance Publique-Hôpitaux de Paris, Hôpital Foch, Service de Neurochirurgie, Institut National de la Santé et de la Recherche Médicale, Université Paris-Sud 11, Hôpital de Bicêtre, Service d'Endocrinologie et des Maladies de la Reproduction, Le Kremlin-Bicêtre F-94275, France
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Assistance Publique-Hôpitaux de Paris, Institut National de la Santé et de la Recherche Médicale, Institut de Cancérologie Gustave-Roussy, Centre National de la Recherche Scientifique, Département de Génomique Fonctionnelle, Assistance Publique-Hôpitaux de Paris, Hôpital Foch, Service de Neurochirurgie, Institut National de la Santé et de la Recherche Médicale, Université Paris-Sud 11, Hôpital de Bicêtre, Service d'Endocrinologie et des Maladies de la Reproduction, Le Kremlin-Bicêtre F-94275, France
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Assistance Publique-Hôpitaux de Paris, Institut National de la Santé et de la Recherche Médicale, Institut de Cancérologie Gustave-Roussy, Centre National de la Recherche Scientifique, Département de Génomique Fonctionnelle, Assistance Publique-Hôpitaux de Paris, Hôpital Foch, Service de Neurochirurgie, Institut National de la Santé et de la Recherche Médicale, Université Paris-Sud 11, Hôpital de Bicêtre, Service d'Endocrinologie et des Maladies de la Reproduction, Le Kremlin-Bicêtre F-94275, France
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Assistance Publique-Hôpitaux de Paris, Institut National de la Santé et de la Recherche Médicale, Institut de Cancérologie Gustave-Roussy, Centre National de la Recherche Scientifique, Département de Génomique Fonctionnelle, Assistance Publique-Hôpitaux de Paris, Hôpital Foch, Service de Neurochirurgie, Institut National de la Santé et de la Recherche Médicale, Université Paris-Sud 11, Hôpital de Bicêtre, Service d'Endocrinologie et des Maladies de la Reproduction, Le Kremlin-Bicêtre F-94275, France
Assistance Publique-Hôpitaux de Paris, Institut National de la Santé et de la Recherche Médicale, Institut de Cancérologie Gustave-Roussy, Centre National de la Recherche Scientifique, Département de Génomique Fonctionnelle, Assistance Publique-Hôpitaux de Paris, Hôpital Foch, Service de Neurochirurgie, Institut National de la Santé et de la Recherche Médicale, Université Paris-Sud 11, Hôpital de Bicêtre, Service d'Endocrinologie et des Maladies de la Reproduction, Le Kremlin-Bicêtre F-94275, France
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Assistance Publique-Hôpitaux de Paris, Institut National de la Santé et de la Recherche Médicale, Institut de Cancérologie Gustave-Roussy, Centre National de la Recherche Scientifique, Département de Génomique Fonctionnelle, Assistance Publique-Hôpitaux de Paris, Hôpital Foch, Service de Neurochirurgie, Institut National de la Santé et de la Recherche Médicale, Université Paris-Sud 11, Hôpital de Bicêtre, Service d'Endocrinologie et des Maladies de la Reproduction, Le Kremlin-Bicêtre F-94275, France
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Assistance Publique-Hôpitaux de Paris, Institut National de la Santé et de la Recherche Médicale, Institut de Cancérologie Gustave-Roussy, Centre National de la Recherche Scientifique, Département de Génomique Fonctionnelle, Assistance Publique-Hôpitaux de Paris, Hôpital Foch, Service de Neurochirurgie, Institut National de la Santé et de la Recherche Médicale, Université Paris-Sud 11, Hôpital de Bicêtre, Service d'Endocrinologie et des Maladies de la Reproduction, Le Kremlin-Bicêtre F-94275, France
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Assistance Publique-Hôpitaux de Paris, Institut National de la Santé et de la Recherche Médicale, Institut de Cancérologie Gustave-Roussy, Centre National de la Recherche Scientifique, Département de Génomique Fonctionnelle, Assistance Publique-Hôpitaux de Paris, Hôpital Foch, Service de Neurochirurgie, Institut National de la Santé et de la Recherche Médicale, Université Paris-Sud 11, Hôpital de Bicêtre, Service d'Endocrinologie et des Maladies de la Reproduction, Le Kremlin-Bicêtre F-94275, France
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Assistance Publique-Hôpitaux de Paris, Institut National de la Santé et de la Recherche Médicale, Institut de Cancérologie Gustave-Roussy, Centre National de la Recherche Scientifique, Département de Génomique Fonctionnelle, Assistance Publique-Hôpitaux de Paris, Hôpital Foch, Service de Neurochirurgie, Institut National de la Santé et de la Recherche Médicale, Université Paris-Sud 11, Hôpital de Bicêtre, Service d'Endocrinologie et des Maladies de la Reproduction, Le Kremlin-Bicêtre F-94275, France
Assistance Publique-Hôpitaux de Paris, Institut National de la Santé et de la Recherche Médicale, Institut de Cancérologie Gustave-Roussy, Centre National de la Recherche Scientifique, Département de Génomique Fonctionnelle, Assistance Publique-Hôpitaux de Paris, Hôpital Foch, Service de Neurochirurgie, Institut National de la Santé et de la Recherche Médicale, Université Paris-Sud 11, Hôpital de Bicêtre, Service d'Endocrinologie et des Maladies de la Reproduction, Le Kremlin-Bicêtre F-94275, France
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cRNA from the reference pool. The arrays were then washed with 0.6× and 0.01× SSC buffers containing Triton, and were dried with a nitrogen gun before scanning with an Agilent DNA microarray scanner. The fluorescence images thus obtained were quantified
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(red) in breast carcinoma cells grown on glass coverslips coated with LN-1 (A) or pG-LN-1 (B) for 1 h. Panel C shows higher magnified image of filopodia (red boxed area in panel B). Overlay of individual fluorescence images was carried out using
Translational Ovarian Cancer Research Program, Department of Anatomy and Cell Biology, Department of Biochemistry, Department of Obstetrics and Gynaecology, Department of Oncology, London Regional Cancer Program, 790 Commissioners Road East, Room A4-836, London, Ontario, Canada N6A 4L6
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Translational Ovarian Cancer Research Program, Department of Anatomy and Cell Biology, Department of Biochemistry, Department of Obstetrics and Gynaecology, Department of Oncology, London Regional Cancer Program, 790 Commissioners Road East, Room A4-836, London, Ontario, Canada N6A 4L6
Translational Ovarian Cancer Research Program, Department of Anatomy and Cell Biology, Department of Biochemistry, Department of Obstetrics and Gynaecology, Department of Oncology, London Regional Cancer Program, 790 Commissioners Road East, Room A4-836, London, Ontario, Canada N6A 4L6
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Translational Ovarian Cancer Research Program, Department of Anatomy and Cell Biology, Department of Biochemistry, Department of Obstetrics and Gynaecology, Department of Oncology, London Regional Cancer Program, 790 Commissioners Road East, Room A4-836, London, Ontario, Canada N6A 4L6
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Translational Ovarian Cancer Research Program, Department of Anatomy and Cell Biology, Department of Biochemistry, Department of Obstetrics and Gynaecology, Department of Oncology, London Regional Cancer Program, 790 Commissioners Road East, Room A4-836, London, Ontario, Canada N6A 4L6
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Translational Ovarian Cancer Research Program, Department of Anatomy and Cell Biology, Department of Biochemistry, Department of Obstetrics and Gynaecology, Department of Oncology, London Regional Cancer Program, 790 Commissioners Road East, Room A4-836, London, Ontario, Canada N6A 4L6
Translational Ovarian Cancer Research Program, Department of Anatomy and Cell Biology, Department of Biochemistry, Department of Obstetrics and Gynaecology, Department of Oncology, London Regional Cancer Program, 790 Commissioners Road East, Room A4-836, London, Ontario, Canada N6A 4L6
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Translational Ovarian Cancer Research Program, Department of Anatomy and Cell Biology, Department of Biochemistry, Department of Obstetrics and Gynaecology, Department of Oncology, London Regional Cancer Program, 790 Commissioners Road East, Room A4-836, London, Ontario, Canada N6A 4L6
Translational Ovarian Cancer Research Program, Department of Anatomy and Cell Biology, Department of Biochemistry, Department of Obstetrics and Gynaecology, Department of Oncology, London Regional Cancer Program, 790 Commissioners Road East, Room A4-836, London, Ontario, Canada N6A 4L6
Translational Ovarian Cancer Research Program, Department of Anatomy and Cell Biology, Department of Biochemistry, Department of Obstetrics and Gynaecology, Department of Oncology, London Regional Cancer Program, 790 Commissioners Road East, Room A4-836, London, Ontario, Canada N6A 4L6
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Translational Ovarian Cancer Research Program, Department of Anatomy and Cell Biology, Department of Biochemistry, Department of Obstetrics and Gynaecology, Department of Oncology, London Regional Cancer Program, 790 Commissioners Road East, Room A4-836, London, Ontario, Canada N6A 4L6
Translational Ovarian Cancer Research Program, Department of Anatomy and Cell Biology, Department of Biochemistry, Department of Obstetrics and Gynaecology, Department of Oncology, London Regional Cancer Program, 790 Commissioners Road East, Room A4-836, London, Ontario, Canada N6A 4L6
Translational Ovarian Cancer Research Program, Department of Anatomy and Cell Biology, Department of Biochemistry, Department of Obstetrics and Gynaecology, Department of Oncology, London Regional Cancer Program, 790 Commissioners Road East, Room A4-836, London, Ontario, Canada N6A 4L6
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Fluor 568-conjugated phalloidin (A12380, Invitrogen). Coverslips were mounted in VectaShield mounting medium (Vector Laboratories) containing 4′,6-diamidino-2-phenylindole (1:1000; Sigma). Fluorescence images of mounted coverslips were captured using a
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:10,000) and fluorescence images of the lower FluoroBlok membrane were taken using an Olympus IX70 microscope with a 4× objective. Determination of the number of migrated or invaded cells was performed using ImageJ software and normalized to the number of cells