Search Results

You are looking at 1 - 2 of 2 items for

  • Author: Arnold D K Hill x
  • Refine by access: All content x
Clear All Modify Search
Marie McIlroy
Search for other papers by Marie McIlroy in
Google Scholar
PubMed
Close
,
Fergal J Fleming Department of Surgery, Department of Surgery, Drug Safety Research Unit, Royal College of Surgeons in Ireland, Dublin 2, Ireland

Search for other papers by Fergal J Fleming in
Google Scholar
PubMed
Close
,
Yvonne Buggy Department of Surgery, Department of Surgery, Drug Safety Research Unit, Royal College of Surgeons in Ireland, Dublin 2, Ireland

Search for other papers by Yvonne Buggy in
Google Scholar
PubMed
Close
,
Arnold D K Hill
Search for other papers by Arnold D K Hill in
Google Scholar
PubMed
Close
, and
Leonie S Young
Search for other papers by Leonie S Young in
Google Scholar
PubMed
Close
Free access
Marie Mc Ilroy
Search for other papers by Marie Mc Ilroy in
Google Scholar
PubMed
Close
,
Fergal J Fleming
Search for other papers by Fergal J Fleming in
Google Scholar
PubMed
Close
,
Yvonne Buggy
Search for other papers by Yvonne Buggy in
Google Scholar
PubMed
Close
,
Arnold D K Hill
Search for other papers by Arnold D K Hill in
Google Scholar
PubMed
Close
, and
Leonie S Young
Search for other papers by Leonie S Young in
Google Scholar
PubMed
Close

Differential signalling between the two oestrogen receptor (ER) isoforms in the presence of tamoxifen has been described. We hypothesise that differential recruitment of the steroid receptor co-activator, SRC-3 to ER-α and ER-β may in part explain associations between ER isoforms and response to endocrine treatment. SRC-3 was localised within epithelial cells of breast tumour tissue and was co-localised with ER-α and ER-β, (n = 112). Expression of SRC-3 was found to be positively associated with ER-α (P = 0.0021) and inversely with ER-β (P < 0.0001). Uniquely, this study utilises primary cell cultures derived from patient tumours, thus providing samples not readily available in most molecular model systems. These samples have enabled us to investigate the influence of growth factor pathways on steroid receptor-co-activator interactions. In HER2 (human epidermal growth factor receptor 2) positive primary tumour cell cultures 17β-estradiol induced a decrease in SRC-3, whereas upregulated SRC-3 expression. Furthermore, treatment with tamoxifen-induced SRC-3 recruitment to the ER-oestrogen response element and enhanced interaction between SRC-3 and ER-α, but not ER-β. Knockdown of SRC-3 results in a concomitant loss of expression of the oestrogen target gene pS2. Furthermore, silencing of SRC-3 resensitizes endocrine resistant, HER2 positive cells to the anti-proliferative effects of tamoxifen. The ability of ER-α, but not ER-β to recruit SRC-3 in the presence of tamoxifen may in part explain the differential ER isoform associations with recurrence in human breast cancer.

Free access