Filter

Refine by date

—

Refine by access

  • Print
  • Save

Search Results

You are looking at 1 - 1 of 1 items for

  • Author: Ryan Ellis x
  • Refine by access: All content x
Clear All Modify Search
Myriem Boufraqech Endocrine Oncology Branch Laboratory of Pathology National Cancer Institute, National Institutes of Health, Center for Cancer Research, Bethesda, Maryland 20892, USA

Search for other papers by Myriem Boufraqech in
Google Scholar
PubMed Close
,
Lisa Zhang Endocrine Oncology Branch Laboratory of Pathology National Cancer Institute, National Institutes of Health, Center for Cancer Research, Bethesda, Maryland 20892, USA

Search for other papers by Lisa Zhang in
Google Scholar
PubMed Close
,
Meenu Jain Endocrine Oncology Branch Laboratory of Pathology National Cancer Institute, National Institutes of Health, Center for Cancer Research, Bethesda, Maryland 20892, USA

Search for other papers by Meenu Jain in
Google Scholar
PubMed Close
,
Dhaval Patel Endocrine Oncology Branch Laboratory of Pathology National Cancer Institute, National Institutes of Health, Center for Cancer Research, Bethesda, Maryland 20892, USA

Search for other papers by Dhaval Patel in
Google Scholar
PubMed Close
,
Ryan Ellis Endocrine Oncology Branch Laboratory of Pathology National Cancer Institute, National Institutes of Health, Center for Cancer Research, Bethesda, Maryland 20892, USA

Search for other papers by Ryan Ellis in
Google Scholar
PubMed Close
,
Yin Xiong Endocrine Oncology Branch Laboratory of Pathology National Cancer Institute, National Institutes of Health, Center for Cancer Research, Bethesda, Maryland 20892, USA

Search for other papers by Yin Xiong in
Google Scholar
PubMed Close
,
Mei He Endocrine Oncology Branch Laboratory of Pathology National Cancer Institute, National Institutes of Health, Center for Cancer Research, Bethesda, Maryland 20892, USA

Search for other papers by Mei He in
Google Scholar
PubMed Close
,
Naris Nilubol Endocrine Oncology Branch Laboratory of Pathology National Cancer Institute, National Institutes of Health, Center for Cancer Research, Bethesda, Maryland 20892, USA

Search for other papers by Naris Nilubol in
Google Scholar
PubMed Close
,
Maria J Merino Endocrine Oncology Branch Laboratory of Pathology National Cancer Institute, National Institutes of Health, Center for Cancer Research, Bethesda, Maryland 20892, USA

Search for other papers by Maria J Merino in
Google Scholar
PubMed Close
, and
Electron Kebebew Endocrine Oncology Branch Laboratory of Pathology National Cancer Institute, National Institutes of Health, Center for Cancer Research, Bethesda, Maryland 20892, USA

Search for other papers by Electron Kebebew in
Google Scholar
PubMed Close

The expression and function of miR-145 in thyroid cancer is unknown. We evaluated the expression and function of miR-145 in thyroid cancer and its potential clinical application as a biomarker. We found that the expression of miR-145 is significantly downregulated in thyroid cancer as compared with normal. Overexpression of miR-145 in thyroid cancer cell lines resulted in: decreased cell proliferation, migration, invasion, VEGF secretion, and E-cadherin expression. miR-145 overexpression also inhibited the PI3K/Akt pathway and directly targeted AKT3. In vivo, miR-145 overexpression decreased tumor growth and metastasis in a xenograft mouse model, and VEGF secretion. miR-145 inhibition in normal primary follicular thyroid cells decreased the expression of thyroid cell differentiation markers. Analysis of indeterminate fine-needle aspiration samples showed miR-145 had a 92% negative predictive value for distinguishing benign from malignant thyroid nodules. Circulating miR-145 levels were significantly higher in patients with thyroid cancer and showed a venous gradient. Serum exosome extractions revealed that miR-145 is secreted. Our findings suggest that miR-145 is a master regulator of thyroid cancer growth, mediates its effect through the PI3K/Akt pathway, is secreted by the thyroid cancer cells, and may serve as an adjunct biomarker for thyroid cancer diagnosis.

Article Type:
Research Article
DOI:
https://doi.org/10.1530/ERC-14-0077
Volume/Issue:
Volume 21: Issue 4
Free access