Search Results

You are looking at 1 - 1 of 1 items for

  • Author: Sofia de Oliveira x
  • Refine by access: All content x
Clear All Modify Search
Cassia Michael Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, Bronx, New York, USA

Search for other papers by Cassia Michael in
Google Scholar
PubMed
Close
,
Juliana Moreira Mendonça-Gomes Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, Bronx, New York, USA

Search for other papers by Juliana Moreira Mendonça-Gomes in
Google Scholar
PubMed
Close
,
Clinton Walton DePaolo Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, Bronx, New York, USA

Search for other papers by Clinton Walton DePaolo in
Google Scholar
PubMed
Close
,
Antonio Di Cristofano Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, Bronx, New York, USA
Department of Medicine (Oncology), Albert Einstein College of Medicine, Bronx, New York, USA
Montefiore-Einstein Comprehensive Cancer Research Center, Albert Einstein College of Medicine, Bronx, New York, USA
Cancer Dormancy Tumor Microenvironment Institute, Albert Einstein College of Medicine, Bronx, New York, USA

Search for other papers by Antonio Di Cristofano in
Google Scholar
PubMed
Close
, and
Sofia de Oliveira Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, Bronx, New York, USA
Montefiore-Einstein Comprehensive Cancer Research Center, Albert Einstein College of Medicine, Bronx, New York, USA
Cancer Dormancy Tumor Microenvironment Institute, Albert Einstein College of Medicine, Bronx, New York, USA
Department of Medicine (Hepatology), Albert Einstein College of Medicine, Bronx, New York, USA
Marion Bessin Liver Research Center, Albert Einstein College of Medicine and Montefiore Medical Center, Bronx, New York, USA

Search for other papers by Sofia de Oliveira in
Google Scholar
PubMed
Close

Anaplastic thyroid cancer (ATC) is of the most aggressive thyroid cancer. While ATC is rare, it accounts for a disproportionately high number of thyroid cancer-related deaths. Here, we developed an ATC xenotransplant model in zebrafish larvae, where we can study tumorigenesis and therapeutic response in vivo. Using both mouse (T4888M) and human (C643)-derived fluorescently labeled ATC cell lines, we show these cell lines display different engraftment rates, mass volume, proliferation, cell death, angiogenic potential, and neutrophil and macrophage recruitment and infiltration. Next, using a PIP-FUCCI reporter to track proliferation in vivo, we observed cells in each phase of the cell cycle. Additionally, we performed long-term non-invasive intravital microscopy over 48 h to understand cellular dynamics in the tumor microenvironment at the single-cell level. Lastly, we tested two drug treatments, AZD2014 and a combination therapy of dabrafenib and trametinib, to show our model could be used as an effective screening platform for new therapeutic compounds for ATC. Altogether, we show that zebrafish xenotransplants make a great model to study thyroid carcinogenesis and the tumor microenvironment, while also being a suitable model to test new therapeutics in vivo.

Restricted access