Search Results

You are looking at 1 - 4 of 4 items for

  • Author: Weiwei Zhou x
  • Refine by access: All content x
Clear All Modify Search
WenQi Yuan Department of Endocrine and Metabolic Diseases, Laboratory of Endocrine and Metabolic Diseases, Division of Endocrine and Metabolic Diseases, School of Medicine, Shanghai Clinical Center for Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai JiaoTong University, 197 Ruijin Er Lu, Shanghai 200025, People's Republic of China
Department of Endocrine and Metabolic Diseases, Laboratory of Endocrine and Metabolic Diseases, Division of Endocrine and Metabolic Diseases, School of Medicine, Shanghai Clinical Center for Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai JiaoTong University, 197 Ruijin Er Lu, Shanghai 200025, People's Republic of China

Search for other papers by WenQi Yuan in
Google Scholar
PubMed
Close
,
WeiQinq Wang Department of Endocrine and Metabolic Diseases, Laboratory of Endocrine and Metabolic Diseases, Division of Endocrine and Metabolic Diseases, School of Medicine, Shanghai Clinical Center for Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai JiaoTong University, 197 Ruijin Er Lu, Shanghai 200025, People's Republic of China
Department of Endocrine and Metabolic Diseases, Laboratory of Endocrine and Metabolic Diseases, Division of Endocrine and Metabolic Diseases, School of Medicine, Shanghai Clinical Center for Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai JiaoTong University, 197 Ruijin Er Lu, Shanghai 200025, People's Republic of China

Search for other papers by WeiQinq Wang in
Google Scholar
PubMed
Close
,
Bin Cui Department of Endocrine and Metabolic Diseases, Laboratory of Endocrine and Metabolic Diseases, Division of Endocrine and Metabolic Diseases, School of Medicine, Shanghai Clinical Center for Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai JiaoTong University, 197 Ruijin Er Lu, Shanghai 200025, People's Republic of China
Department of Endocrine and Metabolic Diseases, Laboratory of Endocrine and Metabolic Diseases, Division of Endocrine and Metabolic Diseases, School of Medicine, Shanghai Clinical Center for Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai JiaoTong University, 197 Ruijin Er Lu, Shanghai 200025, People's Republic of China

Search for other papers by Bin Cui in
Google Scholar
PubMed
Close
,
TingWei Su Department of Endocrine and Metabolic Diseases, Laboratory of Endocrine and Metabolic Diseases, Division of Endocrine and Metabolic Diseases, School of Medicine, Shanghai Clinical Center for Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai JiaoTong University, 197 Ruijin Er Lu, Shanghai 200025, People's Republic of China

Search for other papers by TingWei Su in
Google Scholar
PubMed
Close
,
Yan Ge Department of Endocrine and Metabolic Diseases, Laboratory of Endocrine and Metabolic Diseases, Division of Endocrine and Metabolic Diseases, School of Medicine, Shanghai Clinical Center for Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai JiaoTong University, 197 Ruijin Er Lu, Shanghai 200025, People's Republic of China
Department of Endocrine and Metabolic Diseases, Laboratory of Endocrine and Metabolic Diseases, Division of Endocrine and Metabolic Diseases, School of Medicine, Shanghai Clinical Center for Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai JiaoTong University, 197 Ruijin Er Lu, Shanghai 200025, People's Republic of China

Search for other papers by Yan Ge in
Google Scholar
PubMed
Close
,
Lei Jiang Department of Endocrine and Metabolic Diseases, Laboratory of Endocrine and Metabolic Diseases, Division of Endocrine and Metabolic Diseases, School of Medicine, Shanghai Clinical Center for Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai JiaoTong University, 197 Ruijin Er Lu, Shanghai 200025, People's Republic of China

Search for other papers by Lei Jiang in
Google Scholar
PubMed
Close
,
WeiWei Zhou Department of Endocrine and Metabolic Diseases, Laboratory of Endocrine and Metabolic Diseases, Division of Endocrine and Metabolic Diseases, School of Medicine, Shanghai Clinical Center for Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai JiaoTong University, 197 Ruijin Er Lu, Shanghai 200025, People's Republic of China

Search for other papers by WeiWei Zhou in
Google Scholar
PubMed
Close
, and
Guang Ning Department of Endocrine and Metabolic Diseases, Laboratory of Endocrine and Metabolic Diseases, Division of Endocrine and Metabolic Diseases, School of Medicine, Shanghai Clinical Center for Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai JiaoTong University, 197 Ruijin Er Lu, Shanghai 200025, People's Republic of China
Department of Endocrine and Metabolic Diseases, Laboratory of Endocrine and Metabolic Diseases, Division of Endocrine and Metabolic Diseases, School of Medicine, Shanghai Clinical Center for Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai JiaoTong University, 197 Ruijin Er Lu, Shanghai 200025, People's Republic of China
Department of Endocrine and Metabolic Diseases, Laboratory of Endocrine and Metabolic Diseases, Division of Endocrine and Metabolic Diseases, School of Medicine, Shanghai Clinical Center for Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai JiaoTong University, 197 Ruijin Er Lu, Shanghai 200025, People's Republic of China

Search for other papers by Guang Ning in
Google Scholar
PubMed
Close

To analyze the genetic alterations of pheochromocytomas and evaluate the difference among malignant, extra-adrenal, and benign pheochromocytomas. Forty-three tumor samples were tested for genetic changes using multiplex ligation-dependent probe amplification. Among them, 39 samples were available for protein expression analysis by immunohistochemistry (IHC). All 43 patients (24 women and 19 men; mean age 44.6±13.6 years; range 18–75 years; 9 with malignant, 7 extra-adrenal, and 27 benign) showed multiple copy number losses or gains. The average copy number change was 13.10 in malignant, 13.93 in benign, and 13.47 in paraganglioma patients. There is no significant difference among the three groups of pheochromocytomas. However, we discovered that in the malignant pheochromocytomas, 6 of the 9 patients (67%) showed erythroblastic leukemia viral oncogene homolog 2 (ERBB-2) oncogene gain, whereas only 12 of the 34 (35%) identified change in the benign and extra-adrenal pheochromocytomas. Further, IHC confirmed that ERBB-2-positive staining was more frequent and stronger in malignant pheochromocytomas than in benign and extra-adrenal pheochromocytomas. Our study illustrates the chromosomal changes of the whole genome of Chinese pheochromocytoma patients. The results suggest that there may be certain progression of genetic events that involves chromosomes 1p, 3p, 6p, 11q, 12q, 17q, and 19q in the development of pheochromocytomas, and the activation of ERBB-2 located on chromosome 17q is an important and early event in the malignancy development of these tumor types. The overexpression of ERBB-2 identified by IHC suggested that this oncogene could be associated with the malignancy of pheochromocytomas and paragangliomas.

Open access
Yuling Mao Program of Molecular Medicine, Affiliated Guangzhou Women and Children’s Medical Center, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China
Department of Reproductive Medicine Center, Key Laboratory for Reproductive Medicine of Guangdong Province, Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China

Search for other papers by Yuling Mao in
Google Scholar
PubMed
Close
,
Liuqing Zhu Program of Molecular Medicine, Affiliated Guangzhou Women and Children’s Medical Center, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China
Department of Biochemistry, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China

Search for other papers by Liuqing Zhu in
Google Scholar
PubMed
Close
,
Zhijian Huang Department of Biochemistry, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China

Search for other papers by Zhijian Huang in
Google Scholar
PubMed
Close
,
Chuanghua Luo Department of Biochemistry, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China

Search for other papers by Chuanghua Luo in
Google Scholar
PubMed
Close
,
Ti Zhou Department of Biochemistry, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China

Search for other papers by Ti Zhou in
Google Scholar
PubMed
Close
,
Lei Li Department of Reproductive Medicine Center, Key Laboratory for Reproductive Medicine of Guangdong Province, Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China

Search for other papers by Lei Li in
Google Scholar
PubMed
Close
,
Guannan Wang Department of Biochemistry, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China
Sun Yat-sen University Cancer Center, Guangzhou, China

Search for other papers by Guannan Wang in
Google Scholar
PubMed
Close
,
Zhonghan Yang Department of Biochemistry, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China

Search for other papers by Zhonghan Yang in
Google Scholar
PubMed
Close
,
Weiwei Qi Program of Molecular Medicine, Affiliated Guangzhou Women and Children’s Medical Center, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China
Department of Biochemistry, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China

Search for other papers by Weiwei Qi in
Google Scholar
PubMed
Close
,
Xia Yang Program of Molecular Medicine, Affiliated Guangzhou Women and Children’s Medical Center, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China
Department of Biochemistry, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China
China Key Laboratory of Tropical Disease Control (Sun Yat-sen University), Ministry of Education, Guangzhou, China

Search for other papers by Xia Yang in
Google Scholar
PubMed
Close
, and
Guoquan Gao Program of Molecular Medicine, Affiliated Guangzhou Women and Children’s Medical Center, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China
Department of Biochemistry, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China
Guangdong Engineering & Technology Research Center for Gene Manipulation and Biomacromolecular Products (Sun Yat-sen University), Guangzhou, China
Guangdong Province Key Laboratory of Brain Function and Disease, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China

Search for other papers by Guoquan Gao in
Google Scholar
PubMed
Close

Sorafenib, a small-molecule tyrosine kinase inhibitor with antiangiogenic activity, has been used in liver cancer and kidney cancer treatments. However, clinical trials with sorafenib for breast cancer were stopped in phase III due to limited efficacy. The existence of heterogeneous vasculatures involving tumor cells, such as vessel-like structures formed by vasculogenic mimicry and mosaic vessels, and their resistance to antiangiogenic therapy are thought to be a possible reason for failure of sorafenib therapy. Nevertheless, the features and mechanism of vasculogenesis by tumor cells remain unclear. In the present study, we found that breast cancer stem-like cells (BCSLCs, ALDH1+ cells) were involved in vasculogenic mimicry and mosaic vessel formation in triple-negative breast cancer tissues. Further, only ALDH1+ BCSLCs sorted from MDA-MB-231 could exhibit the tube formation and angiogenesis ability. Sorafenib could inhibit vascularization from endothelial cells rather than that from ALDH1+ cells. α-SMA was identified as a key molecule in vascular formation of BCSLCs. Mechanistically, HIF-1α enhanced the mRNA and protein levels of α-SMA by binding to the HRE element in the promoter directly and meanwhile increased the BCSLCs population. Interestingly, pigment epithelium-derived factor (PEDF), an endogenous angiogenesis inhibitor, could inhibit both endothelial cell-derived and tumor cell-derived angiogenesis by downregulating HIF-1α in breast cancer. Our finding clarified the possible reason for the poor outcome of anti-angiogenesis therapy and PEDF may have the therapeutic potential.

Restricted access
Luming Wu Department of Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People’s Republic of China

Search for other papers by Luming Wu in
Google Scholar
PubMed
Close
,
Jing Xie Department of Pathology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People’s Republic of China

Search for other papers by Jing Xie in
Google Scholar
PubMed
Close
,
Yan Qi Department of Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People’s Republic of China

Search for other papers by Yan Qi in
Google Scholar
PubMed
Close
,
Tingwei Su Department of Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People’s Republic of China

Search for other papers by Tingwei Su in
Google Scholar
PubMed
Close
,
Lei Jiang Department of Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People’s Republic of China

Search for other papers by Lei Jiang in
Google Scholar
PubMed
Close
,
Weiwei Zhou Department of Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People’s Republic of China

Search for other papers by Weiwei Zhou in
Google Scholar
PubMed
Close
,
Yiran Jiang Department of Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People’s Republic of China

Search for other papers by Yiran Jiang in
Google Scholar
PubMed
Close
,
Cui Zhang Department of Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People’s Republic of China

Search for other papers by Cui Zhang in
Google Scholar
PubMed
Close
,
Xu Zhong Department of Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People’s Republic of China

Search for other papers by Xu Zhong in
Google Scholar
PubMed
Close
,
Yanan Cao Department of Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People’s Republic of China

Search for other papers by Yanan Cao in
Google Scholar
PubMed
Close
, and
Weiqing Wang Department of Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People’s Republic of China
Shanghai National Clinical Research Center for Metabolic Diseases, Key Laboratory for Endocrine and Metabolic Diseases of the National Health Commission of the PR China, Shanghai Key Laboratory for Endocrine Tumor, State Key Laboratory of Medical Genomics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China

Search for other papers by Weiqing Wang in
Google Scholar
PubMed
Close

Adrenal incidentalomas are the most frequent human neoplasms. Recent genomic investigations on functional adrenocortical tumors have demonstrated that somatic mutations in PRKACA and KCNJ5 responsible for the development of adrenocortical adenomas (ACAs) are associated with hypercortisolism and aldosteronism, respectively. Several studies have identified CTNNB1 mutations in ACAs and have been mostly involved in the tumorigenesis of non-functional ACA (NFACA). However, integrated genomic characterization of NFACAs is lacking. In the current study, we utilized pan-genomic methods to comprehensively analyze 60 NFACA samples. A total of 1264 somatic mutations in coding regions among the 60 samples were identified, with a median of 15 non-silent mutations per tumor. Twenty-two NFACAs (36.67%) had genetic alterations in CTNNB1. We also identified several somatic mutations in genes of the cAMP/PKA pathway and KCNJ5. Histone modification genes (KMT2A, KMT2C, and KMT2D) were altered in 10% of cases. Germline mutations of MEN1 and RET were also found. Finally, by comparison of our transcriptome data with those available in the TCGA, we illustrated the molecular characterization of NFACA. We revealed the genetic profiling and molecular landscape of NFACA. Wnt/β-catenin pathway activation as shown ssby nuclear and/or cytoplasmic β-catenin accumulation is frequent, occurring in about one–third of ACA cases. cytochrome P450 enzymes could be markers to reveal the functional status of adrenocortical tumors. These observations strongly suggest the involvement of the Wnt/β-catenin pathway in benign adrenal tumorigenesis and possibly in the regulation of steroid secretion.

Restricted access
Luming Wu Department of Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People’s Republic of China

Search for other papers by Luming Wu in
Google Scholar
PubMed
Close
,
Jing Xie Department of Pathology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People’s Republic of China

Search for other papers by Jing Xie in
Google Scholar
PubMed
Close
,
Yan Qi Department of Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People’s Republic of China

Search for other papers by Yan Qi in
Google Scholar
PubMed
Close
,
Tingwei Su Department of Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People’s Republic of China

Search for other papers by Tingwei Su in
Google Scholar
PubMed
Close
,
Lei Jiang Department of Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People’s Republic of China

Search for other papers by Lei Jiang in
Google Scholar
PubMed
Close
,
Weiwei Zhou Department of Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People’s Republic of China

Search for other papers by Weiwei Zhou in
Google Scholar
PubMed
Close
,
Yiran Jiang Department of Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People’s Republic of China

Search for other papers by Yiran Jiang in
Google Scholar
PubMed
Close
,
Cui Zhang Department of Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People’s Republic of China

Search for other papers by Cui Zhang in
Google Scholar
PubMed
Close
,
Xu Zhong Department of Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People’s Republic of China

Search for other papers by Xu Zhong in
Google Scholar
PubMed
Close
,
Yanan Cao Department of Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People’s Republic of China

Search for other papers by Yanan Cao in
Google Scholar
PubMed
Close
, and
Weiqing Wang Department of Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People’s Republic of China
Shanghai National Clinical Research Center for Metabolic Diseases, Key Laboratory for Endocrine and Metabolic Diseases of the National Health Commission of the PR China, Shanghai Key Laboratory for Endocrine Tumor, State Key Laboratory of Medical Genomics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China

Search for other papers by Weiqing Wang in
Google Scholar
PubMed
Close
Free access