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Audrey Ziverec Cancer Research Centre of Lyon (CRCL), INSERM U1052, CNRS UMR5286, Claude Bernard University, Lyon, France

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Marie Chanal Cancer Research Centre of Lyon (CRCL), INSERM U1052, CNRS UMR5286, Claude Bernard University, Lyon, France

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Perrine Raymond Cancer Research Centre of Lyon (CRCL), INSERM U1052, CNRS UMR5286, Claude Bernard University, Lyon, France

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Mirela Diana Ilie Cancer Research Centre of Lyon (CRCL), INSERM U1052, CNRS UMR5286, Claude Bernard University, Lyon, France
Endocrinology Department, “C.I. Parhon” National Institute of Endocrinology, Bucharest, Romania

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Dario De Alcubierre Cancer Research Centre of Lyon (CRCL), INSERM U1052, CNRS UMR5286, Claude Bernard University, Lyon, France

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Arja Pasternack Department of Physiology, Faculty of Medicine, University of Helsinki, Helsinki, Finland

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Olli Ritvos Department of Physiology, Faculty of Medicine, University of Helsinki, Helsinki, Finland

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Gerald Raverot Cancer Research Centre of Lyon (CRCL), INSERM U1052, CNRS UMR5286, Claude Bernard University, Lyon, France
Faculté de Médecine Lyon Est, Université Lyon 1, Lyon, France
Department of Endocrinology, Reference center for rare pituitary disease (HYPO), Groupement Hospitalier EST, Hospices Civils de Lyon, University of Lyon, Lyon, France

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Philippe Bertolino Cancer Research Centre of Lyon (CRCL), INSERM U1052, CNRS UMR5286, Claude Bernard University, Lyon, France

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’-AGGACATAGGGGAGCAGTTTCAG-3’), Ki67 (Fw 5’-GCTGTCCTCAAGACAATCATCA-3’; Rev 5’-GGCGTTATCCCAGGAGACT-3’), Gdf9 (Fw 5’-CAACCAGGTGACAGGACCG-3’; Rev 5’-AGCAAGTGTTCCATGGCAGT-3’), Vegfa (Fw 5’-CTGCTCTCTTGGGTGCACTG-3’; Rev 5’-GCAGCCTGGGACCACTTG-3’), Vegfb (Fw 5

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B Shan
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J Gerez Neuroendocrinology Group, Laboratorio de Fisiología y Biología Molecular, Neurosurgical Clinic, Department of Neurosurgery, Max Planck Institute of Psychiatry, Kraepelinstrasse 10, D-80804 Munich, Germany

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M Haedo Neuroendocrinology Group, Laboratorio de Fisiología y Biología Molecular, Neurosurgical Clinic, Department of Neurosurgery, Max Planck Institute of Psychiatry, Kraepelinstrasse 10, D-80804 Munich, Germany

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M Fuertes Neuroendocrinology Group, Laboratorio de Fisiología y Biología Molecular, Neurosurgical Clinic, Department of Neurosurgery, Max Planck Institute of Psychiatry, Kraepelinstrasse 10, D-80804 Munich, Germany

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M Theodoropoulou
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M Buchfelder Neuroendocrinology Group, Laboratorio de Fisiología y Biología Molecular, Neurosurgical Clinic, Department of Neurosurgery, Max Planck Institute of Psychiatry, Kraepelinstrasse 10, D-80804 Munich, Germany

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M Losa Neuroendocrinology Group, Laboratorio de Fisiología y Biología Molecular, Neurosurgical Clinic, Department of Neurosurgery, Max Planck Institute of Psychiatry, Kraepelinstrasse 10, D-80804 Munich, Germany

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G K Stalla
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E Arzt Neuroendocrinology Group, Laboratorio de Fisiología y Biología Molecular, Neurosurgical Clinic, Department of Neurosurgery, Max Planck Institute of Psychiatry, Kraepelinstrasse 10, D-80804 Munich, Germany

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U Renner
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. Further expanding tumours show cellular hypoxia which leads to a rise in HIF-1 protein expression leading to the production of angiogenic factors, such as vascular endothelial growth factor-A (VEGF-A), which is involved in the sprouting of new vessels into

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Tsai-Der Chuang Department of Obstetrics and Gynecology, University of Florida, Gainesville, Florida 32610-0294, USA

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Harekrushna Panda Department of Obstetrics and Gynecology, University of Florida, Gainesville, Florida 32610-0294, USA

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Xiaoping Luo Department of Obstetrics and Gynecology, University of Florida, Gainesville, Florida 32610-0294, USA

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Nasser Chegini Department of Obstetrics and Gynecology, University of Florida, Gainesville, Florida 32610-0294, USA

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exposure. We confirmed ZEB 1/2 , validated VEGFA, FBLN5 , and TIMP2 as direct targets of miR-200c in MSMC, LSMC, and SKLM-S1, and showed that overexpression of miR-200c caused phenotypic modification of MSMC and LSMC, but not SKLM-S1, and inhibited

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N Garcia de la Torre
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I Buley
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J A H Wass
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H E Turner
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stimuli for angiogenesis has yielded numerous candidates including vascular endothelial growth factor A (VEGF-A), fibroblast growth factor-2 (FGF-2), transforming growth factor α (TGF-α), TGF-β, hepatocyte growth factor, tumour necrosis factor α

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Sonja Balthasar Department of Biology, Turku Graduate School of Biomedical Sciences, Laboratory of Animal Physiology, Hospital for Children and Adolescents, Minerva Foundation Institute for Medical Research, Åbo Akademi University, Tykistökatu 6A, 20520 Turku, Finland

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Nina Bergelin Department of Biology, Turku Graduate School of Biomedical Sciences, Laboratory of Animal Physiology, Hospital for Children and Adolescents, Minerva Foundation Institute for Medical Research, Åbo Akademi University, Tykistökatu 6A, 20520 Turku, Finland
Department of Biology, Turku Graduate School of Biomedical Sciences, Laboratory of Animal Physiology, Hospital for Children and Adolescents, Minerva Foundation Institute for Medical Research, Åbo Akademi University, Tykistökatu 6A, 20520 Turku, Finland

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Christoffer Löf Department of Biology, Turku Graduate School of Biomedical Sciences, Laboratory of Animal Physiology, Hospital for Children and Adolescents, Minerva Foundation Institute for Medical Research, Åbo Akademi University, Tykistökatu 6A, 20520 Turku, Finland

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Minna Vainio Department of Biology, Turku Graduate School of Biomedical Sciences, Laboratory of Animal Physiology, Hospital for Children and Adolescents, Minerva Foundation Institute for Medical Research, Åbo Akademi University, Tykistökatu 6A, 20520 Turku, Finland

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Sture Andersson Department of Biology, Turku Graduate School of Biomedical Sciences, Laboratory of Animal Physiology, Hospital for Children and Adolescents, Minerva Foundation Institute for Medical Research, Åbo Akademi University, Tykistökatu 6A, 20520 Turku, Finland

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Kid Törnquist Department of Biology, Turku Graduate School of Biomedical Sciences, Laboratory of Animal Physiology, Hospital for Children and Adolescents, Minerva Foundation Institute for Medical Research, Åbo Akademi University, Tykistökatu 6A, 20520 Turku, Finland
Department of Biology, Turku Graduate School of Biomedical Sciences, Laboratory of Animal Physiology, Hospital for Children and Adolescents, Minerva Foundation Institute for Medical Research, Åbo Akademi University, Tykistökatu 6A, 20520 Turku, Finland

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amounts of both VEGF-A and -C compared with FRO cells, but S1P stimulates VEGF-A secretion in both cell lines. We show that VEGFR-2 signalling is involved in S1P-induced Akt phosphorylation and migration of ML-1 cells. Furthermore, VEGFR-2 regulates S1P

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Chiara Verdelli Laboratory of Experimental Endocrinology, IRCCS Istituto Ortopedico Galeazzi, Milan, Italy

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Irene Forno Department of Pathophysiology and Organ Transplantation, University of Milan, Milan, Italy

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Annamaria Morotti Department of Pathophysiology and Organ Transplantation, University of Milan, Milan, Italy
Endocrine Surgery, IRCCS Ospedale San Raffaele, Milan, Italy

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Riccardo Maggiore Endocrine Surgery, IRCCS Istituto Auxologico Italiano, Milan, Italy

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Gilberto Mari Endocrine Surgery, IRCCS Istituto Auxologico Italiano, Milan, Italy

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Leonardo Vicentini Division of Pathology, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Milan, Italy

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Stefano Ferrero Endocrine Surgery, IRCCS Ospedale San Raffaele, Milan, Italy
Department of Biomedical, Surgical and Dental Sciences, University of Milan, Milan, Italy

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Elisabetta Kuhn Endocrine Surgery, IRCCS Ospedale San Raffaele, Milan, Italy
Department of Biomedical, Surgical and Dental Sciences, University of Milan, Milan, Italy

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Valentina Vaira Department of Pathophysiology and Organ Transplantation, University of Milan, Milan, Italy
Division of Pathology, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Milan, Italy

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Sabrina Corbetta Department of Biomedical, Surgical and Dental Sciences, University of Milan, Milan, Italy
Endocrinology and Diabetology Service, IRCCS Istituto Ortopedico Galeazzi, Milan, Italy

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expressed at the surface of endothelial cells. One well-known activator of angiogenesis is the secreted protein vascular endothelial growth factor A (VEGFA) ( Ferrara 2009 ). We previously demonstrated that the tumor-associated myofibroblasts (TAMs) are

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Haleh Vosgha Cancer Molecular Pathology, School of Medicine, Menzies Health Institute Queensland, Griffith University, Gold Coast, Queensland, Australia

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Armin Ariana Cancer Molecular Pathology, School of Medicine, Menzies Health Institute Queensland, Griffith University, Gold Coast, Queensland, Australia

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Robert Anthony Smith Cancer Molecular Pathology, School of Medicine, Menzies Health Institute Queensland, Griffith University, Gold Coast, Queensland, Australia
Genomics Research Centre, Institute of Health and Biomedical Innovation, Faculty of Health, Queensland University of Technology, Brisbane, Queensland, Australia

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Alfred King-yin Lam Cancer Molecular Pathology, School of Medicine, Menzies Health Institute Queensland, Griffith University, Gold Coast, Queensland, Australia

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plays an important role in activating the proteins and pro-angiogenic factors involved ( Fig. 1 ). Vascular endothelial growth factor A ( VEGF-A ), above all other known pro-angiogenic factors, induces sprouting of new blood vessels. In thyroid

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C Verdelli Laboratory of Molecular Biology, Department of Human Pathology, Laboratory of Stem Cells for Tissue Engineering, Medical Genetics, Endocrinology Unit, Endocrine Surgery, Surgery Unit, Surgery Unit, Department of Health Sciences, Endocrinology and Diabetology Unit, Endocrinology and Diabetology Unit, IRCCS Policlinico San Donato, San Donato Milanese, Milan, Italy

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L Avagliano Laboratory of Molecular Biology, Department of Human Pathology, Laboratory of Stem Cells for Tissue Engineering, Medical Genetics, Endocrinology Unit, Endocrine Surgery, Surgery Unit, Surgery Unit, Department of Health Sciences, Endocrinology and Diabetology Unit, Endocrinology and Diabetology Unit, IRCCS Policlinico San Donato, San Donato Milanese, Milan, Italy

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P Creo Laboratory of Molecular Biology, Department of Human Pathology, Laboratory of Stem Cells for Tissue Engineering, Medical Genetics, Endocrinology Unit, Endocrine Surgery, Surgery Unit, Surgery Unit, Department of Health Sciences, Endocrinology and Diabetology Unit, Endocrinology and Diabetology Unit, IRCCS Policlinico San Donato, San Donato Milanese, Milan, Italy

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V Guarnieri Laboratory of Molecular Biology, Department of Human Pathology, Laboratory of Stem Cells for Tissue Engineering, Medical Genetics, Endocrinology Unit, Endocrine Surgery, Surgery Unit, Surgery Unit, Department of Health Sciences, Endocrinology and Diabetology Unit, Endocrinology and Diabetology Unit, IRCCS Policlinico San Donato, San Donato Milanese, Milan, Italy

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A Scillitani Laboratory of Molecular Biology, Department of Human Pathology, Laboratory of Stem Cells for Tissue Engineering, Medical Genetics, Endocrinology Unit, Endocrine Surgery, Surgery Unit, Surgery Unit, Department of Health Sciences, Endocrinology and Diabetology Unit, Endocrinology and Diabetology Unit, IRCCS Policlinico San Donato, San Donato Milanese, Milan, Italy

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L Vicentini Laboratory of Molecular Biology, Department of Human Pathology, Laboratory of Stem Cells for Tissue Engineering, Medical Genetics, Endocrinology Unit, Endocrine Surgery, Surgery Unit, Surgery Unit, Department of Health Sciences, Endocrinology and Diabetology Unit, Endocrinology and Diabetology Unit, IRCCS Policlinico San Donato, San Donato Milanese, Milan, Italy

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G B Steffano Laboratory of Molecular Biology, Department of Human Pathology, Laboratory of Stem Cells for Tissue Engineering, Medical Genetics, Endocrinology Unit, Endocrine Surgery, Surgery Unit, Surgery Unit, Department of Health Sciences, Endocrinology and Diabetology Unit, Endocrinology and Diabetology Unit, IRCCS Policlinico San Donato, San Donato Milanese, Milan, Italy

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E Beretta Laboratory of Molecular Biology, Department of Human Pathology, Laboratory of Stem Cells for Tissue Engineering, Medical Genetics, Endocrinology Unit, Endocrine Surgery, Surgery Unit, Surgery Unit, Department of Health Sciences, Endocrinology and Diabetology Unit, Endocrinology and Diabetology Unit, IRCCS Policlinico San Donato, San Donato Milanese, Milan, Italy

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L Soldati Laboratory of Molecular Biology, Department of Human Pathology, Laboratory of Stem Cells for Tissue Engineering, Medical Genetics, Endocrinology Unit, Endocrine Surgery, Surgery Unit, Surgery Unit, Department of Health Sciences, Endocrinology and Diabetology Unit, Endocrinology and Diabetology Unit, IRCCS Policlinico San Donato, San Donato Milanese, Milan, Italy

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E Costa Laboratory of Molecular Biology, Department of Human Pathology, Laboratory of Stem Cells for Tissue Engineering, Medical Genetics, Endocrinology Unit, Endocrine Surgery, Surgery Unit, Surgery Unit, Department of Health Sciences, Endocrinology and Diabetology Unit, Endocrinology and Diabetology Unit, IRCCS Policlinico San Donato, San Donato Milanese, Milan, Italy

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A Spada Laboratory of Molecular Biology, Department of Human Pathology, Laboratory of Stem Cells for Tissue Engineering, Medical Genetics, Endocrinology Unit, Endocrine Surgery, Surgery Unit, Surgery Unit, Department of Health Sciences, Endocrinology and Diabetology Unit, Endocrinology and Diabetology Unit, IRCCS Policlinico San Donato, San Donato Milanese, Milan, Italy

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G P Bulfamante Laboratory of Molecular Biology, Department of Human Pathology, Laboratory of Stem Cells for Tissue Engineering, Medical Genetics, Endocrinology Unit, Endocrine Surgery, Surgery Unit, Surgery Unit, Department of Health Sciences, Endocrinology and Diabetology Unit, Endocrinology and Diabetology Unit, IRCCS Policlinico San Donato, San Donato Milanese, Milan, Italy

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S Corbetta Laboratory of Molecular Biology, Department of Human Pathology, Laboratory of Stem Cells for Tissue Engineering, Medical Genetics, Endocrinology Unit, Endocrine Surgery, Surgery Unit, Surgery Unit, Department of Health Sciences, Endocrinology and Diabetology Unit, Endocrinology and Diabetology Unit, IRCCS Policlinico San Donato, San Donato Milanese, Milan, Italy

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FAM dye-labelled (vascular endothelial growth factor A ( VEGFA ), Hs000900055_m1; α -SMA , Hs00426835_g1; PTH , Hs00757710_1g and CCND1 , Hs00765553_m1, Applied Biosystems, Life Technologies), 10 μl of 2× TaqMan Gene Expression MasterMix (Applied

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Charline Dubois Laboratory of Tumour and Development Biology, GIGA-Cancer, University of Liège, CHU-B23, Liège, Belgium

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Natacha Rocks Laboratory of Tumour and Development Biology, GIGA-Cancer, University of Liège, CHU-B23, Liège, Belgium

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Silvia Blacher Laboratory of Tumour and Development Biology, GIGA-Cancer, University of Liège, CHU-B23, Liège, Belgium

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Irina Primac Laboratory of Tumour and Development Biology, GIGA-Cancer, University of Liège, CHU-B23, Liège, Belgium

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Anne Gallez Laboratory of Tumour and Development Biology, GIGA-Cancer, University of Liège, CHU-B23, Liège, Belgium

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Melissa García-Caballero Laboratory of Tumour and Development Biology, GIGA-Cancer, University of Liège, CHU-B23, Liège, Belgium

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Céline Gérard Laboratory of Tumour and Development Biology, GIGA-Cancer, University of Liège, CHU-B23, Liège, Belgium

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Laurent Brouchet Thoracic Surgery Department, University Hospital CHU Toulouse, Toulouse, France

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Agnès Noël Laboratory of Tumour and Development Biology, GIGA-Cancer, University of Liège, CHU-B23, Liège, Belgium

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Françoise Lenfant INSERM UMR1048, Institut des Maladies Métaboliques et Cardiovasculaires – I2MC, University of Toulouse III Paul Sabatier, UPS, Toulouse, France

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Didier Cataldo Laboratory of Tumour and Development Biology, GIGA-Cancer, University of Liège, CHU-B23, Liège, Belgium

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Christel Pequeux Laboratory of Tumour and Development Biology, GIGA-Cancer, University of Liège, CHU-B23, Liège, Belgium

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, when OVX females were used, E2 treatment increased tumour-related blood vessel network, while no modulation was observed in males. VEGFC, VEGFD, VEGFA and bFGF, the main prolymphangiogenic and proangiogenic factors, were measured by Milliplex assay in

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Zara Zelenko Division of Endocrinology, Diabetes and Bone Diseases, Icahn School of Medicine at Mount Sinai, New York, New York, USA

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Emily Jane Gallagher Division of Endocrinology, Diabetes and Bone Diseases, Icahn School of Medicine at Mount Sinai, New York, New York, USA

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Irini Markella Antoniou Division of Endocrinology, Diabetes and Bone Diseases, Icahn School of Medicine at Mount Sinai, New York, New York, USA

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Deepali Sachdev Department of Medicine and Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota, USA

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Anupma Nayak Department of Pathology and Laboratory Medicine, The Mount Sinai Hospital and Icahn School of Medicine at Mount Sinai, New York, New York, USA

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Douglas Yee Department of Medicine and Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota, USA

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Derek LeRoith Division of Endocrinology, Diabetes and Bone Diseases, Icahn School of Medicine at Mount Sinai, New York, New York, USA

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′CAGAGGATGACCTGCCAACA; 3′GCCCTTCCTTTCCTGTGTCAT. SLUG primers: 5′TTTCTGGGCTGGCCAAACAT; 3′TTCTCCCCCGTGTGAGTTCTA. TWIST1 primers: 5′GCCGGAGACCTAGATGTCATT; 3′CCCACGCCCTGTTTCTTTGA. VEGFA primers: 5′AGGCCAGCACATAGGAGAGA; 3′TTTCTTGCGCTTTCGTTTTT. VEGFD primers: 5

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