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T Frogne Department of Tumor Endocrinology, Institute of Cancer Biology, Danish Cancer Society, Strandboulevarden 49, DK-2100 Copenhagen, Denmark

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J S Jepsen Department of Tumor Endocrinology, Institute of Cancer Biology, Danish Cancer Society, Strandboulevarden 49, DK-2100 Copenhagen, Denmark

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S S Larsen Department of Tumor Endocrinology, Institute of Cancer Biology, Danish Cancer Society, Strandboulevarden 49, DK-2100 Copenhagen, Denmark

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C K Fog Department of Tumor Endocrinology, Institute of Cancer Biology, Danish Cancer Society, Strandboulevarden 49, DK-2100 Copenhagen, Denmark

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B L Brockdorff Department of Tumor Endocrinology, Institute of Cancer Biology, Danish Cancer Society, Strandboulevarden 49, DK-2100 Copenhagen, Denmark

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A E Lykkesfeldt Department of Tumor Endocrinology, Institute of Cancer Biology, Danish Cancer Society, Strandboulevarden 49, DK-2100 Copenhagen, Denmark

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resistance in sublines of MCF-7 human breast cancer cells. Cancer Research 57 585 –589. Molloy CA , May FE & Westley BR 2000 Insulin receptor substrate-1 expression is regulated by estrogen in the MCF-7 human breast cancer

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Cindy M Staka Tenovus Centre for Cancer Research, Welsh School of Pharmacy, Cardiff University, Redwood Building, King Edward VII Avenue, Cardiff, UK

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Robert I Nicholson Tenovus Centre for Cancer Research, Welsh School of Pharmacy, Cardiff University, Redwood Building, King Edward VII Avenue, Cardiff, UK

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Julia M W Gee Tenovus Centre for Cancer Research, Welsh School of Pharmacy, Cardiff University, Redwood Building, King Edward VII Avenue, Cardiff, UK

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using mouse xenografts of aromatase-transfected MCF-7Ca breast cancer cells ( Brodie et al. 2003 ). Long-term oestrogen deprived ER+ in vitro models ( Martin et al. 2003 , Santen et al. 2004 ) have also been developed from endocrine responsive

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Penn Muluhngwi Department of Biochemistry and Molecular Genetics, Center for Genetics and Molecular Medicine, University of Louisville School of Medicine, Louisville, Kentucky 40292, USA

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Carolyn M Klinge Department of Biochemistry and Molecular Genetics, Center for Genetics and Molecular Medicine, University of Louisville School of Medicine, Louisville, Kentucky 40292, USA

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, 48, and 72 h; 50 nM 4-OHT, 0, 3, and 5 min T47D, TAM-R MCF7 vs TAM-S MCF7 Enhanced expression in TAM-R MCF7 cells ( Lu et al . 2011 ) Anti-miR-181b suppressed TAM-R xenograft tumor growth in TAM treated mice TIMP3 (L, W, Q) ( Lu et al . 2011

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Glenn T G Chang
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Mila Jhamai
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Wytske M van Weerden
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Guido Jenster
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Albert O Brinkmann
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normal fetal calf serum (FCS) and characterised as described by Horoszewicz et al. (1983) and Chang et al. (1997) . Human androgen-independent prostate cancer cell lines (DU145 and PC3) and human breast cancer cell lines (ZR-75-1, MCF-7, T-47D, SK

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Zhaoxia Zhang Department of Physiology and Cell Biology, The Ohio State Biochemistry Graduate Program, The Integrated Biomedical Science Graduate Program, The Ohio State University, 304 Hamilton Hall, 1645 Neil Avenue, Columbus, Ohio 43210, USA
Department of Physiology and Cell Biology, The Ohio State Biochemistry Graduate Program, The Integrated Biomedical Science Graduate Program, The Ohio State University, 304 Hamilton Hall, 1645 Neil Avenue, Columbus, Ohio 43210, USA

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Sasha Beyer Department of Physiology and Cell Biology, The Ohio State Biochemistry Graduate Program, The Integrated Biomedical Science Graduate Program, The Ohio State University, 304 Hamilton Hall, 1645 Neil Avenue, Columbus, Ohio 43210, USA
Department of Physiology and Cell Biology, The Ohio State Biochemistry Graduate Program, The Integrated Biomedical Science Graduate Program, The Ohio State University, 304 Hamilton Hall, 1645 Neil Avenue, Columbus, Ohio 43210, USA

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Sissy M Jhiang Department of Physiology and Cell Biology, The Ohio State Biochemistry Graduate Program, The Integrated Biomedical Science Graduate Program, The Ohio State University, 304 Hamilton Hall, 1645 Neil Avenue, Columbus, Ohio 43210, USA
Department of Physiology and Cell Biology, The Ohio State Biochemistry Graduate Program, The Integrated Biomedical Science Graduate Program, The Ohio State University, 304 Hamilton Hall, 1645 Neil Avenue, Columbus, Ohio 43210, USA
Department of Physiology and Cell Biology, The Ohio State Biochemistry Graduate Program, The Integrated Biomedical Science Graduate Program, The Ohio State University, 304 Hamilton Hall, 1645 Neil Avenue, Columbus, Ohio 43210, USA

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pERK levels, an indicator of MEK/ERK activation, in human breast tumors. Taken together, MEK activation appears to play an important role in maintaining NIS protein stability in human breast cancers. Materials and methods Cell culture MCF-7 breast

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Takashi Suzuki
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Akio Inoue
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Yasuhiro Miki
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Takuya Moriya
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Jun-ichi Akahira
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Takanori Ishida
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Hisashi Hirakawa
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Yuri Yamaguchi
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Shin-ichi Hayashi
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Hironobu Sasano
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. 5F ) of these three cells were not significantly altered between the absence and presence of doxycyclin for 3 days. Morphological features of Egr3-expressing MCF-7 cells in athymic mice xenograft model In

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M A Redondo-Müller School of Biological and Chemical Sciences, Queen Mary, University of London, Mile End Road, London E1 4NS, UK

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M Stevanovic-Walker School of Biological and Chemical Sciences, Queen Mary, University of London, Mile End Road, London E1 4NS, UK

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S Barker School of Biological and Chemical Sciences, Queen Mary, University of London, Mile End Road, London E1 4NS, UK

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J R Puddefoot School of Biological and Chemical Sciences, Queen Mary, University of London, Mile End Road, London E1 4NS, UK

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G P Vinson School of Biological and Chemical Sciences, Queen Mary, University of London, Mile End Road, London E1 4NS, UK

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). Body weights, as an index of toxicity, showed no change in these groups ( Fig. 4 D), and there was no mortality. In vivo xenograft assay Confirmation of the hollow fibre cell data was sought in xenograft experiments, and because MCF-7 cells responded

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T M Sadler Department of Oncology, Wyeth Research, 401 North Middle Town Road, Pearl River, New York, New York 10965, USA

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M Gavriil Department of Oncology, Wyeth Research, 401 North Middle Town Road, Pearl River, New York, New York 10965, USA

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T Annable Department of Oncology, Wyeth Research, 401 North Middle Town Road, Pearl River, New York, New York 10965, USA

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P Frost Department of Oncology, Wyeth Research, 401 North Middle Town Road, Pearl River, New York, New York 10965, USA

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L M Greenberger Department of Oncology, Wyeth Research, 401 North Middle Town Road, Pearl River, New York, New York 10965, USA

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Y Zhang Department of Oncology, Wyeth Research, 401 North Middle Town Road, Pearl River, New York, New York 10965, USA

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arrest. Our preliminary experiments also showed dramatic anti-tumor activity against an MCF-7 xenograft model. Furthermore, at the molecular level, we demonstrated that the observed synergistic effect was likely due to the combination effect on several

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Céline Van Themsche Research Group in Molecular Oncology and Endocrinology, Department of Chemistry and Biology, Canada Research Chair in Molecular Gyneco-Oncology, Université du Québec à Trois-Rivières, 3351, Boul. des Forges, CP 500, Trois-Rivières, Québec, Canada G9A 5H7

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Sophie Parent Research Group in Molecular Oncology and Endocrinology, Department of Chemistry and Biology, Canada Research Chair in Molecular Gyneco-Oncology, Université du Québec à Trois-Rivières, 3351, Boul. des Forges, CP 500, Trois-Rivières, Québec, Canada G9A 5H7

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Valérie Leblanc Research Group in Molecular Oncology and Endocrinology, Department of Chemistry and Biology, Canada Research Chair in Molecular Gyneco-Oncology, Université du Québec à Trois-Rivières, 3351, Boul. des Forges, CP 500, Trois-Rivières, Québec, Canada G9A 5H7

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Caroline Descôteaux Research Group in Molecular Oncology and Endocrinology, Department of Chemistry and Biology, Canada Research Chair in Molecular Gyneco-Oncology, Université du Québec à Trois-Rivières, 3351, Boul. des Forges, CP 500, Trois-Rivières, Québec, Canada G9A 5H7

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Anne-Marie Simard Research Group in Molecular Oncology and Endocrinology, Department of Chemistry and Biology, Canada Research Chair in Molecular Gyneco-Oncology, Université du Québec à Trois-Rivières, 3351, Boul. des Forges, CP 500, Trois-Rivières, Québec, Canada G9A 5H7

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Gervais Bérubé Research Group in Molecular Oncology and Endocrinology, Department of Chemistry and Biology, Canada Research Chair in Molecular Gyneco-Oncology, Université du Québec à Trois-Rivières, 3351, Boul. des Forges, CP 500, Trois-Rivières, Québec, Canada G9A 5H7

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Eric Asselin Research Group in Molecular Oncology and Endocrinology, Department of Chemistry and Biology, Canada Research Chair in Molecular Gyneco-Oncology, Université du Québec à Trois-Rivières, 3351, Boul. des Forges, CP 500, Trois-Rivières, Québec, Canada G9A 5H7

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classical xenograft model where human breast cancer cells MCF-7 (ERα-positive) and MDA-MB-468 (ERα-negative) were grown subcutaneously in nude mice to evaluate the biological activity of VP-128 ( Fig. 1 A) in vivo . We found that administration of VP-128 to

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Cécile M Vouyovitch Centre de Recherche en Cancérologie de Lyon, UMR INSERM 1052-CNRS 5286, Centre Léon Bérard, Université Claude Bernard Lyon I, Université de Lyon, Lyon, France

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Jo K Perry Liggins Institute, University of Auckland, Auckland, New Zealand

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Dong Xu Liu Liggins Institute, University of Auckland, Auckland, New Zealand

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Laurent Bezin Centre de Recherche en Neurosciences de Lyon, UMR INSERM U1028-CNRS5292, Université de Lyon, Lyon, France

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Eric Vilain Department of Human Genetics, University of California, Los Angeles, California, USA

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Jean-Jacques Diaz Centre de Recherche en Cancérologie de Lyon, UMR INSERM 1052-CNRS 5286, Centre Léon Bérard, Université Claude Bernard Lyon I, Université de Lyon, Lyon, France

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Peter E Lobie Cancer Science Institute of Singapore and Department of Pharmacology, National University of Singapore, Singapore, Republic of Singapore

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Hichem C Mertani Centre de Recherche en Cancérologie de Lyon, UMR INSERM 1052-CNRS 5286, Centre Léon Bérard, Université Claude Bernard Lyon I, Université de Lyon, Lyon, France

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every 15min for 24h. Xenograft analyses Mammary tumors were established using MCF7-hGH and MCF7-VEC cells as described ( Mukhina et al. 2004 , Zhu et al. 2005 a , Brunet-Dunand et al. 2009 ) with approval from the Animal Ethics Committee

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