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resistance in sublines of MCF-7 human breast cancer cells. Cancer Research 57 585 –589. Molloy CA , May FE & Westley BR 2000 Insulin receptor substrate-1 expression is regulated by estrogen in the MCF-7 human breast cancer
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using mouse xenografts of aromatase-transfected MCF-7Ca breast cancer cells ( Brodie et al. 2003 ). Long-term oestrogen deprived ER+ in vitro models ( Martin et al. 2003 , Santen et al. 2004 ) have also been developed from endocrine responsive
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, 48, and 72 h; 50 nM 4-OHT, 0, 3, and 5 min T47D, TAM-R MCF7 vs TAM-S MCF7 Enhanced expression in TAM-R MCF7 cells ( Lu et al . 2011 ) Anti-miR-181b suppressed TAM-R xenograft tumor growth in TAM treated mice TIMP3 (L, W, Q) ( Lu et al . 2011
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normal fetal calf serum (FCS) and characterised as described by Horoszewicz et al. (1983) and Chang et al. (1997) . Human androgen-independent prostate cancer cell lines (DU145 and PC3) and human breast cancer cell lines (ZR-75-1, MCF-7, T-47D, SK
Department of Physiology and Cell Biology, The Ohio State Biochemistry Graduate Program, The Integrated Biomedical Science Graduate Program, The Ohio State University, 304 Hamilton Hall, 1645 Neil Avenue, Columbus, Ohio 43210, USA
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Department of Physiology and Cell Biology, The Ohio State Biochemistry Graduate Program, The Integrated Biomedical Science Graduate Program, The Ohio State University, 304 Hamilton Hall, 1645 Neil Avenue, Columbus, Ohio 43210, USA
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Department of Physiology and Cell Biology, The Ohio State Biochemistry Graduate Program, The Integrated Biomedical Science Graduate Program, The Ohio State University, 304 Hamilton Hall, 1645 Neil Avenue, Columbus, Ohio 43210, USA
Department of Physiology and Cell Biology, The Ohio State Biochemistry Graduate Program, The Integrated Biomedical Science Graduate Program, The Ohio State University, 304 Hamilton Hall, 1645 Neil Avenue, Columbus, Ohio 43210, USA
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pERK levels, an indicator of MEK/ERK activation, in human breast tumors. Taken together, MEK activation appears to play an important role in maintaining NIS protein stability in human breast cancers. Materials and methods Cell culture MCF-7 breast
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. 5F ) of these three cells were not significantly altered between the absence and presence of doxycyclin for 3 days. Morphological features of Egr3-expressing MCF-7 cells in athymic mice xenograft model In
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). Body weights, as an index of toxicity, showed no change in these groups ( Fig. 4 D), and there was no mortality. In vivo xenograft assay Confirmation of the hollow fibre cell data was sought in xenograft experiments, and because MCF-7 cells responded
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arrest. Our preliminary experiments also showed dramatic anti-tumor activity against an MCF-7 xenograft model. Furthermore, at the molecular level, we demonstrated that the observed synergistic effect was likely due to the combination effect on several
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classical xenograft model where human breast cancer cells MCF-7 (ERα-positive) and MDA-MB-468 (ERα-negative) were grown subcutaneously in nude mice to evaluate the biological activity of VP-128 ( Fig. 1 A) in vivo . We found that administration of VP-128 to
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every 15min for 24h. Xenograft analyses Mammary tumors were established using MCF7-hGH and MCF7-VEC cells as described ( Mukhina et al. 2004 , Zhu et al. 2005 a , Brunet-Dunand et al. 2009 ) with approval from the Animal Ethics Committee