alterations found in TC (reviewed in Muzza et al. 2020 ). We recently developed a PTC-MA assay that able to evaluate in an extremely cost-effective manner (300 euro/sample), a total of 24 genetic alterations including point mutations and fusions frequently
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Carla Colombo, Marina Muzza, Gabriele Pogliaghi, Sonia Palazzo, Guia Vannucchi, Leonardo Vicentini, Luca Persani, Giacomo Gazzano, and Laura Fugazzola
Stéphanie Durand, Carole Ferraro-Peyret, Mireille Joufre, Annie Chave, Françoise Borson-Chazot, Samia Selmi-Ruby, and Bernard Rousset
distinct and larger series of PTC. Transcripts from six genes: C11ORF8 , SDC4 , CHI3L1 , PROS1 , FN1 , and TIMP1 were assayed in 69 PTC (51 PTC-ga(+) and 18 PTC-ga(−)) and in 30 NT samples by quantitative PCR. Among the 51 PTC-ga(+), 41 presented a
Paola Caria, Tinuccia Dettori, Daniela V Frau, Angela Borghero, Antonello Cappai, Alessia Riola, Maria L Lai, Francesco Boi, Piergiorgio Calò, Angelo Nicolosi, Stefano Mariotti, and Roberta Vanni
(Invitrogen/Life Technologies) using a Quant-iT RNA assay kit (Invitrogen/Life Technologies). RET/PTC1 rearrangements were detected by RT-PCR. RT-PCR was followed by agarose gel electrophoresis. Briefly, 20 nM total RNA were reverse transcribed using a
Wei Zhang, Hang Zhang, and Xudong Zhao
thyroid carcinomas ( Pillai et al . 2015 ). Although therapeutic treatments via thyroidectomy or radioactive iodine demonstrate a favorable prognosis for PTC patients in the early stage ( Ma et al . 2018 ), aggressive PTC with larger tumor size, lymph
Cristina Romei, Raffaele Ciampi, Pinuccia Faviana, Laura Agate, Eleonora Molinaro, Valeria Bottici, Fulvio Basolo, Paolo Miccoli, Furio Pacini, Aldo Pinchera, and Rossella Elisei
Introduction Papillary thyroid carcinomas (PTCs) are the most frequent malignant disease of the thyroid gland and are usually well-differentiated, as demonstrated by their ability to take up iodine, to secrete thyroglobulin (Tg), and to be
Yangang Wang, Meiju Ji, Wei Wang, Zhimin Miao, Peng Hou, Xinyan Chen, Feng Xu, Guangwu Zhu, Xianlu Sun, Yujun Li, Steven Condouris, Dingxie Liu, Shengli Yan, Jie Pan, and Mingzhao Xing
inter-assay coefficient of variability being 2.5–5.5% and 5.0–12.0% respectively. Genomic DNA isolation Paraffin-embedded PTC samples were microdissected and DNA isolated as previously described ( Xing et al . 2005 ). Briefly, after an 8-h treatment at
Debora Degl'Innocenti, Paola Romeo, Eva Tarantino, Marialuisa Sensi, Giuliana Cassinelli, Veronica Catalano, Cinzia Lanzi, Federica Perrone, Silvana Pilotti, Ettore Seregni, Marco A Pierotti, Angela Greco, and Maria Grazia Borrello
replicates. Cell migration and invasion assays Forty-eight hours after transfection, PTC cells were harvested and transferred into 24-well transwell chambers (Costar, Corning, Inc., Corning, NY, USA) in complete medium. For the migration assay, cells were
Yang Zhao, Cangang Zhang, Yanan Zhu, Xi Ding, Yikun Zhou, Hongjun Lv, Yuxuan Lin, Yuan Wu, Bingyin Shi, and Jiao Fu
TREM1 in PTCs (T) compared to matched noncancerous tissues (N) by qRT-PCR assay ( n = 32). (B) TREM1 protein level was significantly higher in PTC tissues (T, n = 52) compared to adjacent noncancerous tissues (N, n = 52). (C) Comparison of TREM1
Maria Rosaria Rusciano, Marcella Salzano, Sara Monaco, Maria Rosaria Sapio, Maddalena Illario, Valentina De Falco, Massimo Santoro, Pietro Campiglia, Lucio Pastore, Gianfranco Fenzi, Guido Rossi, and Mario Vitale
, Bedford, MA, USA). After overnight incubation at 4 °C, the plates were washed thrice with PBS and used. Rat CaMKIIα kinase-deficient mutant CaMKIIαK42M was a generous gift of Dr A R Means. Ras V12 , RET/PTC-3, RET/PTC-3 Y1015F , and RET/PTC-3 Y1062F were
Viktoria Evdokimova, Manoj Gandhi, Jayanagendra Rayapureddi, James R Stringer, and Yuri E Nikiforov
different periods, 1, 2, 4, 6, 24, 48, and 72 h, and subjected to γH2AX (H2AFX) immunofluorescence. Electroporation of restriction enzymes and detection of RET/PTC rearrangements PvuII, StuI, EcoRV, ScaI, and NruI RE (New England Bio Labs, Ipswick, MA, USA
