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. 2018 ). Along with the AT release of adipokines that may contribute to tumor progression, extracellular vesicles (EVs) derived from obese AT also play a role in this scenario ( Robado de Lope et al. 2018 ). EVs are small membrane vesicles coated
Department of Plant Pathology and Microbiology, National Taiwan University, Taipei, Taiwan
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isolation, storage, recovery, and the characterization from biofluids, such as plasma, serum, or urine, need to be optimized and standardized for clinical applications. The International Society for Extracellular Vesicles recently provided guidelines for EV
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various malignancies, including PTC ( Khatami & Tavangar 2018 , Rappa et al. 2019 , Toraih et al. 2021 ). Special attention was paid to small non-coding RNAs, micro-RNAs (miRNAs), and small lipid bilayer-enclosed extracellular vesicles (EVs), both
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Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, Ochsner Clinic Foundation, New Orleans, Louisiana, USA
Perinatology Research Branch, NICHD/NIH, Wayne State University, Detroit, Michigan, USA
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Mater Ovarian Cancer Research Collaborative, Mater Adult Hospital, South Brisbane, Queensland, Australia
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Mater Ovarian Cancer Research Collaborative, Mater Adult Hospital, South Brisbane, Queensland, Australia
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Department of Clinical Biochemistry and Immunology, Faculty of Pharmacy, University of Concepción, Concepción, Chile
Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, Ochsner Clinic Foundation, New Orleans, Louisiana, USA
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and metastasis. At the beginning, cancer cells activate the endogenic oncogenic pathways and prompt the cells to proliferate in their local niches. Nevertheless, at some point, these cells recruit endogenous secretions, such as extracellular vesicles
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-tubulin was used as loading control. Western blots analysis were performed in triplicate. (B) Extracellular MIF was endocytosed in vesicle-like structures via CD74. Cells were incubated with 400 ng/ml Alexa 546 MIF for 30 and 60 min at 37 °C. Negligible
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). Exosomes are extracellular vesicles (diameter 30–150 nm) secreted by cells with various biological functions ( Mears et al. 2004 , Sharma et al. 2016 ). Their involvement in intercellular communication has been widely depicted by delivering signaling
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tail ( Fig. 1 ). LRIGs show homology in their extracellular domains and also in the juxtamembrane portion of their cytoplasmic domains. The remainder of the cytoplasmic domain diverges significantly amongst LRIGs ( Abraira et al . 2010 ) and may impart
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). Extracellular vesicles (EVs) derived from metastatic tumor cells can also be detected in the circulation in men with CRPC, including those that are CTC-negative, and may carry tumor-associated proteins that can be quantified and tracked serially ( Gerdtsson et
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transported across the plasma membrane ( Schafer et al. 2004 ) and/or can be released by extracellular vesicle shedding ( Taverna et al. 2003 ). FGF7 is present at approximately 28 ng/g wet weight in normal prostate ( Giri et al. 1999 a ). FGF7 contains
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melanosomes, smooth endoplasmic reticulum, recycling endosomes, neurotransmitter vesicles, mRNA ( Reck-Peterson et al . 2000 , Langford 2002 , Salerno et al . 2008 ), and neuroendocrine vesicles ( Rudolf et al . 2003 ). A role for MYO5A in tumor cell