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Myriem Boufraqech Endocrine Oncology Branch Laboratory of Pathology National Cancer Institute, National Institutes of Health, Center for Cancer Research, Bethesda, Maryland 20892, USA

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Lisa Zhang Endocrine Oncology Branch Laboratory of Pathology National Cancer Institute, National Institutes of Health, Center for Cancer Research, Bethesda, Maryland 20892, USA

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Meenu Jain Endocrine Oncology Branch Laboratory of Pathology National Cancer Institute, National Institutes of Health, Center for Cancer Research, Bethesda, Maryland 20892, USA

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Dhaval Patel Endocrine Oncology Branch Laboratory of Pathology National Cancer Institute, National Institutes of Health, Center for Cancer Research, Bethesda, Maryland 20892, USA

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Ryan Ellis Endocrine Oncology Branch Laboratory of Pathology National Cancer Institute, National Institutes of Health, Center for Cancer Research, Bethesda, Maryland 20892, USA

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Yin Xiong Endocrine Oncology Branch Laboratory of Pathology National Cancer Institute, National Institutes of Health, Center for Cancer Research, Bethesda, Maryland 20892, USA

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Mei He Endocrine Oncology Branch Laboratory of Pathology National Cancer Institute, National Institutes of Health, Center for Cancer Research, Bethesda, Maryland 20892, USA

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Naris Nilubol Endocrine Oncology Branch Laboratory of Pathology National Cancer Institute, National Institutes of Health, Center for Cancer Research, Bethesda, Maryland 20892, USA

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Maria J Merino Endocrine Oncology Branch Laboratory of Pathology National Cancer Institute, National Institutes of Health, Center for Cancer Research, Bethesda, Maryland 20892, USA

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Electron Kebebew Endocrine Oncology Branch Laboratory of Pathology National Cancer Institute, National Institutes of Health, Center for Cancer Research, Bethesda, Maryland 20892, USA

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have demonstrated the role of miRNAs in physiologic and pathologic conditions, including evidence suggesting miRNA dysregulation in tumorigenesis ( Solomides et al . 2012 ). Among the many miRNAs dysregulated in cancer, miR-145 has been proposed to be

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Belinda J Petri Department of Biochemistry & Molecular Genetics, University of Louisville School of Medicine Louisville, Kentucky, USA

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Kellianne M Piell Department of Biochemistry & Molecular Genetics, University of Louisville School of Medicine Louisville, Kentucky, USA

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Ali E Wilt Department of Biochemistry & Molecular Genetics, University of Louisville School of Medicine Louisville, Kentucky, USA

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Alexa D Howser Department of Biochemistry & Molecular Genetics, University of Louisville School of Medicine Louisville, Kentucky, USA

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Laura Winkler Department of Biochemistry & Molecular Genetics, University of Louisville School of Medicine Louisville, Kentucky, USA

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Mattie R Whitworth Department of Biochemistry & Molecular Genetics, University of Louisville School of Medicine Louisville, Kentucky, USA

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Bailey L Valdes Department of Biochemistry & Molecular Genetics, University of Louisville School of Medicine Louisville, Kentucky, USA

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Norman L Lehman Department of Biochemistry & Molecular Genetics, University of Louisville School of Medicine Louisville, Kentucky, USA
Pathology and Laboratory Medicine, University of Louisville, Louisville, Kentucky, USA
The Brown Cancer Center, University of Louisville, Louisville, Kentucky, USA

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Brian F Clem Department of Biochemistry & Molecular Genetics, University of Louisville School of Medicine Louisville, Kentucky, USA
The Brown Cancer Center, University of Louisville, Louisville, Kentucky, USA

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Carolyn M Klinge Department of Biochemistry & Molecular Genetics, University of Louisville School of Medicine Louisville, Kentucky, USA
The Brown Cancer Center, University of Louisville, Louisville, Kentucky, USA
Center for Integrative Environmental Health Sciences (CIEHS), University of Louisville, Louisville, Kentucky, USA

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in TAM-resistant cell lines. We tested the hypothesis that A2B1 downregulates miRNAs that directly target PHGDH and PSAT1 , resulting in increased protein levels. We identified A2B1-regulated miRNAs, miR-145-5p and miR-424-5p, that directly target

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Sheeba Jacob
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S Nayak
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Gwendolyn Fernandes Primate Biology Laboratory, GS Medical College and KEM Hospital, The Centre for Medical Bioinformatics, Department of Pathology, National Institute for Research in Reproductive Health (NIRRH), Indian Council of Medical Research, JM Street, Parel, Mumbai 400012, India

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R S Barai Primate Biology Laboratory, GS Medical College and KEM Hospital, The Centre for Medical Bioinformatics, Department of Pathology, National Institute for Research in Reproductive Health (NIRRH), Indian Council of Medical Research, JM Street, Parel, Mumbai 400012, India

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S Menon Primate Biology Laboratory, GS Medical College and KEM Hospital, The Centre for Medical Bioinformatics, Department of Pathology, National Institute for Research in Reproductive Health (NIRRH), Indian Council of Medical Research, JM Street, Parel, Mumbai 400012, India

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U K Chaudhari
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S D Kholkute
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Geetanjali Sachdeva
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in DU145 and lowest levels in LNCaP cells ( Fig. 4 A, B, C, D, E, F, G and H). This prompted us to in silico explore the presence of androgen response elements (AREs) and ZEB2-binding sites in MIR200A and MIR200B genes. Figure 10 (A) miR200a and

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Xiaoli Liu Division of Thyroid Surgery, China-Japan Union Hospital of Jilin University, Jilin Provincial Key Laboratory of Surgical Translational Medicine, Changchun, Jilin, China

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Chunhai Zhang Division of Thyroid Surgery, China-Japan Union Hospital of Jilin University, Jilin Provincial Key Laboratory of Surgical Translational Medicine, Changchun, Jilin, China

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Xiaomiao Wang Division of Thyroid Surgery, China-Japan Union Hospital of Jilin University, Jilin Provincial Key Laboratory of Surgical Translational Medicine, Changchun, Jilin, China

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Can Cui Division of Thyroid Surgery, China-Japan Union Hospital of Jilin University, Jilin Provincial Key Laboratory of Surgical Translational Medicine, Changchun, Jilin, China

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Hanwen Cui Division of Thyroid Surgery, China-Japan Union Hospital of Jilin University, Jilin Provincial Key Laboratory of Surgical Translational Medicine, Changchun, Jilin, China

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Baishu Zhu Division of Thyroid Surgery, China-Japan Union Hospital of Jilin University, Jilin Provincial Key Laboratory of Surgical Translational Medicine, Changchun, Jilin, China

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Anqi Chen Division of Thyroid Surgery, China-Japan Union Hospital of Jilin University, Jilin Provincial Key Laboratory of Surgical Translational Medicine, Changchun, Jilin, China

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Lu Zhang Division of Thyroid Surgery, China-Japan Union Hospital of Jilin University, Jilin Provincial Key Laboratory of Surgical Translational Medicine, Changchun, Jilin, China

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Jingwei Xin Division of Thyroid Surgery, China-Japan Union Hospital of Jilin University, Jilin Provincial Key Laboratory of Surgical Translational Medicine, Changchun, Jilin, China

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Qingfeng Fu Division of Thyroid Surgery, China-Japan Union Hospital of Jilin University, Jilin Provincial Key Laboratory of Surgical Translational Medicine, Changchun, Jilin, China

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Gianlorenzo Dionigi Department of Pathophysiology and Transplantation, University of Milan, Milan, Italy
Division of Surgery, Istituto Auxologico Italiano, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS), Milan, Italy

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Hui Sun Division of Thyroid Surgery, China-Japan Union Hospital of Jilin University, Jilin Provincial Key Laboratory of Surgical Translational Medicine, Changchun, Jilin, China

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, miR-145-3p and miR-139-5p to disrupt the miRNAs-mediated inhibition of VEGFA and VEGFC and inactivation of transforming growth factor (TGF)-β signaling. Collectively, our results provide experimental evidence regarding the clinical significance and

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Neetu Dahiya Laboratory of Cellular and Molecular Biology, Department of Pathology, National Institute on Aging, NIH Biomedical Research Center, 251 Bayview Boulevard, Suite 100, Room 6C228, Baltimore, Maryland 21224, USA

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Patrice J Morin Laboratory of Cellular and Molecular Biology, Department of Pathology, National Institute on Aging, NIH Biomedical Research Center, 251 Bayview Boulevard, Suite 100, Room 6C228, Baltimore, Maryland 21224, USA
Laboratory of Cellular and Molecular Biology, Department of Pathology, National Institute on Aging, NIH Biomedical Research Center, 251 Bayview Boulevard, Suite 100, Room 6C228, Baltimore, Maryland 21224, USA

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, miR-299 , let7d , miR-155 , miR-140 , miR-222 , miR-337 , miR-124a , miR-99a , miR-331 , miR-104 , miR-150 , miR-184 , miR-152 , miR-145 , miR-424 , miR-224 , miR-302c EOC cell lines versus normal Zhang et al . (2008)  None

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Libero Santarpia Translational Research Unit, Endocrine Neoplasia and Hormonal Disorders, Experimental Therapeutics, Department of Oncology, Hospital of Prato and Istituto Toscana Tumori, Piazza dell' Ospedale, 59100 Prato, Italy Departments of
Translational Research Unit, Endocrine Neoplasia and Hormonal Disorders, Experimental Therapeutics, Department of Oncology, Hospital of Prato and Istituto Toscana Tumori, Piazza dell' Ospedale, 59100 Prato, Italy Departments of

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Milena Nicoloso Translational Research Unit, Endocrine Neoplasia and Hormonal Disorders, Experimental Therapeutics, Department of Oncology, Hospital of Prato and Istituto Toscana Tumori, Piazza dell' Ospedale, 59100 Prato, Italy Departments of

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George A Calin Translational Research Unit, Endocrine Neoplasia and Hormonal Disorders, Experimental Therapeutics, Department of Oncology, Hospital of Prato and Istituto Toscana Tumori, Piazza dell' Ospedale, 59100 Prato, Italy Departments of

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miR-145 are two clustered miRs on chromosome 5q that are commonly downregulated in different tumor types, such as tumors of the colon, breast, and ovary ( Michael et al . 2003 , Iorio et al . 2005 , 2007 , Akao et al . 2007 , Chen et

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Helen C Miller Department of Surgery and Cancer, Imperial College, Hammersmith Hospital Campus, London, UK

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Adam E Frampton Department of Surgery and Cancer, Imperial College, Hammersmith Hospital Campus, London, UK

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Anna Malczewska Department of Surgery and Cancer, Imperial College, Hammersmith Hospital Campus, London, UK
Department of Pathophysiology and Endocrinology, School of Medicine with the Division of Dentistry in Zabrze, Medical University of Silesia, Katowice, Poland

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Silvia Ottaviani Department of Surgery and Cancer, Imperial College, Hammersmith Hospital Campus, London, UK

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Euan A Stronach Department of Surgery and Cancer, Imperial College, Hammersmith Hospital Campus, London, UK

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Rashpal Flora Department of Histopathology, Imperial College Healthcare NHS Trust, Hammersmith Hospital, London, UK

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Daniel Kaemmerer Zentralklinik Bad Berka GmbH, Robert-Koch-Allee, Bad Berka, Germany

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Gert Schwach Institute of Pathophysiology, Center for Molecular Medicine, Medical University of Graz, Graz, Austria

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Roswitha Pfragner Institute of Pathophysiology, Center for Molecular Medicine, Medical University of Graz, Graz, Austria

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Omar Faiz St Mark’s Hospital, Harrow, UK

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Beata Kos-Kudła Department of Pathophysiology and Endocrinology, School of Medicine with the Division of Dentistry in Zabrze, Medical University of Silesia, Katowice, Poland

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George B Hanna Academic Surgical Unit, Department of Surgery and Cancer, Imperial College, St Mary’s Campus, London, UK

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Justin Stebbing Department of Pathophysiology and Endocrinology, School of Medicine with the Division of Dentistry in Zabrze, Medical University of Silesia, Katowice, Poland

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Leandro Castellano Department of Pathophysiology and Endocrinology, School of Medicine with the Division of Dentistry in Zabrze, Medical University of Silesia, Katowice, Poland

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Andrea Frilling Department of Surgery and Cancer, Imperial College, Hammersmith Hospital Campus, London, UK

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.6 0.04581073 miR-548 0.5 0.006746057 miR-191-5p 0.5 0.04581073 miR-145-5p b,c −0.7 0.018028326 miR-1825 c −0.5 0.04792938 miR-1233 c −0.8 0.00034256 miR-331-5p c −0.7 0.0143562 miR-1 c −0.8 0

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Valeria Coppola Department of Hematology, Department of Experimental Oncology, Oncology and Molecular Medicine, Istituto Superiore Sanità, viale Regina Elena 299, 00161 Rome, Italy

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Ruggero De Maria Department of Hematology, Department of Experimental Oncology, Oncology and Molecular Medicine, Istituto Superiore Sanità, viale Regina Elena 299, 00161 Rome, Italy
Department of Hematology, Department of Experimental Oncology, Oncology and Molecular Medicine, Istituto Superiore Sanità, viale Regina Elena 299, 00161 Rome, Italy

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Désirée Bonci Department of Hematology, Department of Experimental Oncology, Oncology and Molecular Medicine, Istituto Superiore Sanità, viale Regina Elena 299, 00161 Rome, Italy

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↑ ↓ ↓ miR-125b ↓ ↓ ↓ ↑ miR-128 ↓ ↓ miR-141 ↑ ↑ miR-143 ↓ ↓ miR-145 ↓ ↓ miR-146 ↑ ↓ miR-148 ↑ ↓ miR-16 ↑ ↓ ↓ ↓ miR-181

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Claire E Fletcher Department of Surgery and Cancer, Cardiff University School of Medicine, Imperial College London, Imperial Centre for Translational and Experimental Medicine, Du Cane Road, London W12 0NN, UK

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D Alwyn Dart Department of Surgery and Cancer, Cardiff University School of Medicine, Imperial College London, Imperial Centre for Translational and Experimental Medicine, Du Cane Road, London W12 0NN, UK
Department of Surgery and Cancer, Cardiff University School of Medicine, Imperial College London, Imperial Centre for Translational and Experimental Medicine, Du Cane Road, London W12 0NN, UK

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Charlotte L Bevan Department of Surgery and Cancer, Cardiff University School of Medicine, Imperial College London, Imperial Centre for Translational and Experimental Medicine, Du Cane Road, London W12 0NN, UK

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et al . 2008 , Rokhlin et al . 2008 , Tong et al . 2008 ). Of these, miR-101, miR-145, miR-34a, miR-182, miR-375, miR-181a, miR-92b and miR-125 were upregulated by androgen, whilst miR-16, miR-126*, miR-23b, miR-100, miR-222, miR-133a, miR-499 and

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O Chabre Institut National de la Santé et de la Recherche Médicale, Department of Endocrinology, Commissariat à l'Energie Atomique, Lab. BCI, Paris Descartes University, Centre National de la Recherche Scientifique UMR 8104, Department of Endocrinology, Unité 1036, 17, Rue des Martyrs, F-38054 Grenoble Cedex 09, France
Institut National de la Santé et de la Recherche Médicale, Department of Endocrinology, Commissariat à l'Energie Atomique, Lab. BCI, Paris Descartes University, Centre National de la Recherche Scientifique UMR 8104, Department of Endocrinology, Unité 1036, 17, Rue des Martyrs, F-38054 Grenoble Cedex 09, France
Institut National de la Santé et de la Recherche Médicale, Department of Endocrinology, Commissariat à l'Energie Atomique, Lab. BCI, Paris Descartes University, Centre National de la Recherche Scientifique UMR 8104, Department of Endocrinology, Unité 1036, 17, Rue des Martyrs, F-38054 Grenoble Cedex 09, France
Institut National de la Santé et de la Recherche Médicale, Department of Endocrinology, Commissariat à l'Energie Atomique, Lab. BCI, Paris Descartes University, Centre National de la Recherche Scientifique UMR 8104, Department of Endocrinology, Unité 1036, 17, Rue des Martyrs, F-38054 Grenoble Cedex 09, France

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R Libé Institut National de la Santé et de la Recherche Médicale, Department of Endocrinology, Commissariat à l'Energie Atomique, Lab. BCI, Paris Descartes University, Centre National de la Recherche Scientifique UMR 8104, Department of Endocrinology, Unité 1036, 17, Rue des Martyrs, F-38054 Grenoble Cedex 09, France
Institut National de la Santé et de la Recherche Médicale, Department of Endocrinology, Commissariat à l'Energie Atomique, Lab. BCI, Paris Descartes University, Centre National de la Recherche Scientifique UMR 8104, Department of Endocrinology, Unité 1036, 17, Rue des Martyrs, F-38054 Grenoble Cedex 09, France
Institut National de la Santé et de la Recherche Médicale, Department of Endocrinology, Commissariat à l'Energie Atomique, Lab. BCI, Paris Descartes University, Centre National de la Recherche Scientifique UMR 8104, Department of Endocrinology, Unité 1036, 17, Rue des Martyrs, F-38054 Grenoble Cedex 09, France

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G Assie Institut National de la Santé et de la Recherche Médicale, Department of Endocrinology, Commissariat à l'Energie Atomique, Lab. BCI, Paris Descartes University, Centre National de la Recherche Scientifique UMR 8104, Department of Endocrinology, Unité 1036, 17, Rue des Martyrs, F-38054 Grenoble Cedex 09, France
Institut National de la Santé et de la Recherche Médicale, Department of Endocrinology, Commissariat à l'Energie Atomique, Lab. BCI, Paris Descartes University, Centre National de la Recherche Scientifique UMR 8104, Department of Endocrinology, Unité 1036, 17, Rue des Martyrs, F-38054 Grenoble Cedex 09, France
Institut National de la Santé et de la Recherche Médicale, Department of Endocrinology, Commissariat à l'Energie Atomique, Lab. BCI, Paris Descartes University, Centre National de la Recherche Scientifique UMR 8104, Department of Endocrinology, Unité 1036, 17, Rue des Martyrs, F-38054 Grenoble Cedex 09, France

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O Barreau Institut National de la Santé et de la Recherche Médicale, Department of Endocrinology, Commissariat à l'Energie Atomique, Lab. BCI, Paris Descartes University, Centre National de la Recherche Scientifique UMR 8104, Department of Endocrinology, Unité 1036, 17, Rue des Martyrs, F-38054 Grenoble Cedex 09, France
Institut National de la Santé et de la Recherche Médicale, Department of Endocrinology, Commissariat à l'Energie Atomique, Lab. BCI, Paris Descartes University, Centre National de la Recherche Scientifique UMR 8104, Department of Endocrinology, Unité 1036, 17, Rue des Martyrs, F-38054 Grenoble Cedex 09, France
Institut National de la Santé et de la Recherche Médicale, Department of Endocrinology, Commissariat à l'Energie Atomique, Lab. BCI, Paris Descartes University, Centre National de la Recherche Scientifique UMR 8104, Department of Endocrinology, Unité 1036, 17, Rue des Martyrs, F-38054 Grenoble Cedex 09, France

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J Bertherat Institut National de la Santé et de la Recherche Médicale, Department of Endocrinology, Commissariat à l'Energie Atomique, Lab. BCI, Paris Descartes University, Centre National de la Recherche Scientifique UMR 8104, Department of Endocrinology, Unité 1036, 17, Rue des Martyrs, F-38054 Grenoble Cedex 09, France
Institut National de la Santé et de la Recherche Médicale, Department of Endocrinology, Commissariat à l'Energie Atomique, Lab. BCI, Paris Descartes University, Centre National de la Recherche Scientifique UMR 8104, Department of Endocrinology, Unité 1036, 17, Rue des Martyrs, F-38054 Grenoble Cedex 09, France
Institut National de la Santé et de la Recherche Médicale, Department of Endocrinology, Commissariat à l'Energie Atomique, Lab. BCI, Paris Descartes University, Centre National de la Recherche Scientifique UMR 8104, Department of Endocrinology, Unité 1036, 17, Rue des Martyrs, F-38054 Grenoble Cedex 09, France

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X Bertagna Institut National de la Santé et de la Recherche Médicale, Department of Endocrinology, Commissariat à l'Energie Atomique, Lab. BCI, Paris Descartes University, Centre National de la Recherche Scientifique UMR 8104, Department of Endocrinology, Unité 1036, 17, Rue des Martyrs, F-38054 Grenoble Cedex 09, France
Institut National de la Santé et de la Recherche Médicale, Department of Endocrinology, Commissariat à l'Energie Atomique, Lab. BCI, Paris Descartes University, Centre National de la Recherche Scientifique UMR 8104, Department of Endocrinology, Unité 1036, 17, Rue des Martyrs, F-38054 Grenoble Cedex 09, France
Institut National de la Santé et de la Recherche Médicale, Department of Endocrinology, Commissariat à l'Energie Atomique, Lab. BCI, Paris Descartes University, Centre National de la Recherche Scientifique UMR 8104, Department of Endocrinology, Unité 1036, 17, Rue des Martyrs, F-38054 Grenoble Cedex 09, France

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J-J Feige Institut National de la Santé et de la Recherche Médicale, Department of Endocrinology, Commissariat à l'Energie Atomique, Lab. BCI, Paris Descartes University, Centre National de la Recherche Scientifique UMR 8104, Department of Endocrinology, Unité 1036, 17, Rue des Martyrs, F-38054 Grenoble Cedex 09, France
Institut National de la Santé et de la Recherche Médicale, Department of Endocrinology, Commissariat à l'Energie Atomique, Lab. BCI, Paris Descartes University, Centre National de la Recherche Scientifique UMR 8104, Department of Endocrinology, Unité 1036, 17, Rue des Martyrs, F-38054 Grenoble Cedex 09, France
Institut National de la Santé et de la Recherche Médicale, Department of Endocrinology, Commissariat à l'Energie Atomique, Lab. BCI, Paris Descartes University, Centre National de la Recherche Scientifique UMR 8104, Department of Endocrinology, Unité 1036, 17, Rue des Martyrs, F-38054 Grenoble Cedex 09, France

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N Cherradi Institut National de la Santé et de la Recherche Médicale, Department of Endocrinology, Commissariat à l'Energie Atomique, Lab. BCI, Paris Descartes University, Centre National de la Recherche Scientifique UMR 8104, Department of Endocrinology, Unité 1036, 17, Rue des Martyrs, F-38054 Grenoble Cedex 09, France
Institut National de la Santé et de la Recherche Médicale, Department of Endocrinology, Commissariat à l'Energie Atomique, Lab. BCI, Paris Descartes University, Centre National de la Recherche Scientifique UMR 8104, Department of Endocrinology, Unité 1036, 17, Rue des Martyrs, F-38054 Grenoble Cedex 09, France
Institut National de la Santé et de la Recherche Médicale, Department of Endocrinology, Commissariat à l'Energie Atomique, Lab. BCI, Paris Descartes University, Centre National de la Recherche Scientifique UMR 8104, Department of Endocrinology, Unité 1036, 17, Rue des Martyrs, F-38054 Grenoble Cedex 09, France

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subjects and ACA or ACC patients. We demonstrate a strong predictive value of low miR-195 and high miR-483-5p circulating levels for the diagnosis and prognosis of aggressive ACC (aACC). Materials and methods Patients and clinical samples Informed signed

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