cDNA and qRT-PCR of miR, TaqMan MicroRNA Assays (Applied Biosystems, Life Technologies) were performed according to the manufacturer's instructions. Representative data are presented as relative fold changes in miR-339-5p levels compared with the U6
Search Results
Aparna Lakshmanan, Anna Wojcicka, Marta Kotlarek, Xiaoli Zhang, Krystian Jazdzewski, and Sissy M Jhiang
Neetu Dahiya and Patrice J Morin
normal ovarian tissues Nam et al . (2008 a ) miR-182 , miR-200c , miR-142-3p , miR-200b , miR-135b , miR-200a , miR-195 , miR-126* , miR-26b , miR-10b , miR-126 , miR-199b-5p , miR-107 , miR-30b , miR-192 , miR-335 , miR-32
Pierlorenzo Pallante, Rosa Visone, Carlo Maria Croce, and Alfredo Fusco
) 192 , 197 , 328 , 346 Up Weber et al . (2006) PDTC 187 , 221 , 129 , 222 , 146b , 339 , 183 Up Nikiforova et al . (2008) ATC 30d , 125b , 26a , 30a-5p Down Visone et al . (2007 b ) 302c , 205
P Pallante, R Visone, M Ferracin, A Ferraro, M T Berlingieri, G Troncone, G Chiappetta, C G Liu, M Santoro, M Negrini, C M Croce, and A Fusco
. References Altuvia Y , Landgraf P, Lithwick G, Elefant N, Pfeffer S, Aravin A, Brownstein MJ, Tuschl T & Margalit H 2005 Clustering and conservation patterns of human microRNAs. Nucleic Acids Research 33 2697 –2706
Goswin Y Meyer-Rochow, Nicole E Jackson, John V Conaglen, Denis E Whittle, Muthusamy Kunnimalaiyaan, Herbert Chen, Gunnar Westin, Johanna Sandgren, Peter Stålberg, Elham Khanafshar, Daniel Shibru, Quan-Yang Duh, Orlo H Clark, Electron Kebebew, Anthony J Gill, Rory Clifton-Bligh, Bruce G Robinson, Diana E Benn, and Stan B Sidhu
LOcally WEighted Scatterplot Smoothing (Lowess) regression algorithm. MiRNA qPCR analysis Differential expression was confirmed in selected miRNAs (miR-15a, miR-16, and miR-483-5p) from total RNA by qPCR using TaqMan miRNA assay probes (Applied Biosystems
Claire E Fletcher, D Alwyn Dart, and Charlotte L Bevan
(KHSRP1), heterogeneous nuclear ribonucleoprotein A1 (hnRNPA1), and the DEAD box RNA helicases, p68 (DDX5) and p72 (DDX17) ( Fukuda et al . 2007 ). The primary function of DGCR8 is to bind to the junction between single-stranded and double
Tsai-Der Chuang, Harekrushna Panda, Xiaoping Luo, and Nasser Chegini
expressed genes were selected based on P ≤0.05 (two-way ANOVA and Tukey's test) and a 1.5-fold change cutoff and subjected to cluster and tree-view and functional analyses using Ingenuity Pathways Software (Ingenuity Systems, Redwood City, CA, USA). For
Yumi Endo, Tatsuya Toyama, Satoru Takahashi, Nobuyasu Yoshimoto, Mai Iwasa, Tomoko Asano, Yoshitaka Fujii, and Hiroko Yamashita
.711±0.201 miR-29c 24.295±0.390 miR-34a 26.339±0.240 mR-129 34.759±0.185 miR-146a 26.160±0.221 miR-193b 21.112±0.219 miR-342-3p 24.456±0.322 miR-1290 27.612±0.445 U6B 27.091±0.154 Table 3 Correlation between expression levels of miRNAs and clinicopathological
Chiara Borga, Carlo Alberto Dal Pozzo, Elisabetta Trevellin, Francesca Bergamo, Sabina Murgioni, Anna Caterina Milanetto, Claudio Pasquali, Umberto Cillo, Giada Munari, Chiara Martini, Eugenio De Carlo, Vittorina Zagonel, Vincenza Guzzardo, Gianmaria Pennelli, Angelo Paolo Dei Tos, Roberto Vettor, and Matteo Fassan
, comparing the tumor grading according to 2019 WHO classification ( WHO Classification of Tumours Editorial Board 2019 ), the somatostatin receptors expression status (SSTR2A and SSTR5) and the activation of the mTOR signaling pathway (ph-mTOR, ph-p70S6K, ph
María Ángeles Castilla, María Ángeles López-García, María Reina Atienza, Juan Manuel Rosa-Rosa, Juan Díaz-Martín, María Luisa Pecero, Begoña Vieites, Laura Romero-Pérez, Javier Benítez, Annarica Calcabrini, and José Palacios
; basal CKs, basal cytokeratins (CK5/6 and/or CK17); TN-NOS, triple-negative not otherwise specified; TN-BL, triple-negative basal-like. * P values were calculated using the Pearson's χ 2 test. Bold, P values are statistically significant. This study